响应面法优化曲霉Asaw117脱色培养基的研究  

STUDY ON OPTIMIZATION OF LIQUID FERMENTATION MEDIUM OF ASAW117 BY RESPONSE SURFACE METHOD

作　　者：梁红昌[1] 张庆华[1] 吴瑜瑜[1] 吴晓玉[1] 

机构地区：[1]南昌市发酵应用技术重点实验室江西农业大学生物科学与工程学院,南昌330045

出　　处：《环境工程》2009年第S1期218-221,241,共5页Environmental Engineering

基　　金：江西省教育厅科技项目(赣教技字〔2005〕100号);江西农业大学研究生创新专项资金项目

摘　　要：采用响应面法对曲霉Asaw117脱色培养基成分进行了优化。首先用Plackett—Burman(PB)设计对培养基中的相关因素的效应进行评价,以脱色率为响应值,筛选出2个可信度>90%以上的硝酸钠和pH作为重要影响因素。然后利用Box-Behnken设计及响应面分析,最终确定培养基的最优组合为:硝酸钠1.39g/L、磷酸氢二钾1.25g/L、氯化钾0.625g/L、硫酸镁0.625g/L、硫酸亚铁0.01g/L、蔗糖50g/L、pH6.37,培养温度为35℃。经过优化以后,曲霉Asaw117在5g/L的染料培养基中的脱色率达到90%,比优化前的51.7%提高了1.74倍。Response surface method(RSM)was used to optimize decolorization medium of Asaw117.Firstly,Plackett-Burman(PB)design was used to evaluate the influence of related factors and choosed decolorization rate as response value.It showed that two factors playing the important roles in the medium were selected,i.e.NaNO_3 and pH,whose credibility was more than 90%.Secondly,the Box-Behnken design and response surface analysis were used to determine the best combination,which is NaNO_3 1.39 g/L,K_2HPO_4 1.25 g/L,KCl 0.625 g/L,MgSO_4 0.625 g/L,FeSO_4 0.01 g/L,sucrose 50 g/L,pH 6.37,culture temperature 35 ℃.After optimization,the decolorization of Asaw117 was increased by 1.74 times from 51.7% to 90.0% using 5 g/L dye medium.

关 键 词：响应面法 脱色培养基 优化 

分 类 号：Q93[生物学—微生物学]