淫羊藿苷对动脉性ED模型大鼠阴茎海绵体压力和一氧化氮合酶亚型表达的影响  

Effects of icariin on the erectile function and expression of nitrogen oxide synthase isoforms in corpus cavernosum of arteriogenic erectile dysfunction rat model

作　　者：田龙[1] 辛钟成[2] 刘武江[2] 袁亦铭[2] 刘刚[2] 陈亮[2] 付杰[2] 王玲珑[1] 

机构地区：[1]武汉大学人民医院泌尿外科,430060 [2]北京大学第一医院泌尿外科北京大学泌尿外科研究所

出　　处：《中华医学杂志》2004年第11期954-957,共4页National Medical Journal of China

基　　金：国家自然科学基金资助项目 ( 3 0 0 70 92 7)

摘　　要：目的　建立动脉性勃起功能障碍大鼠模型 (arteriogenicerectiledysfunction ,A -ED) ,研究淫羊藿苷 (Icariin)对A -ED大鼠勃起功能和一氧化氮合酶 (NOS)三种亚型表达的影响。方法　 4 0只Wistar大鼠随机分为假手术组 (A组 )、A -ED组 (B组 )、A -ED低剂量淫羊藿苷治疗组 (C组 )和AED高剂量淫羊藿苷治疗组 (D组 )。结扎大鼠双侧髂内动脉建立A -ED动物模型后 ,C组和D组每天口饲不同剂量的淫羊藿苷 ( 5mg/kg ,10mg/k) ,A组和B组口饲等体积的生理盐水。 30d后 ,通过检测各组大鼠阴茎海绵体压力 (ICP)变化评估淫羊藿苷对大鼠勃起功能的效果 ,切取阴茎海绵体组织 ,通过逆转录 -PCR和Western印迹方法检测N0S亚型的表达变化。结果　建立A -ED模型 30d后 ,B组ICP较手术前显著降低 ,N0S三种亚型的表达水平也显著降低。经淫羊藿苷治疗后 ,C组和D组的ICP比B组明显升高 (P <0 .0 1) ,且D组较C组增高效果明显 ;C组和D组的eNOS表达水平较之B组有所增高 ,与ICP的变化有相同的趋势。C组的iNOS表达水平有一定程度的升高。治疗后nNOS表达水平没有明显增高。结论　经淫羊藿苷治疗后大鼠勃起功能显著改善 ,这种功能改善与eN0S的表达增加有相同的趋势 ,提示淫羊藿苷可能通过恢复eN0s的表达来改善A -ED模型的勃起功能。Objective The study the effects of oral administered icariin on intracavernosal pressure(ICP) and on expression of the nitrogen oxide synthase (NOS) isoforms in corpus cavernosum (CC) of arteriogenic erectile dysfunction (A-ED) rat model.Methods Forty adult male Wistar rats were randomly divided into 4 groups of 10 rats: shame operated group (group A) and three A-ED model groups (groups B,C and D). The internal pudendal arteries were isolated and ligated with 7-0 nylon thread at both the main trunk and the penile branches to establish the A-ED model. ICP were tested after the operation to make sure the successful model establishment. The groups A and B were treated with saline; and the groups C and Dwere treated with icariin (5mg/kg/day and 10mg/kg/day respectively) orally for 30 days. Then the ICP was measured again. The tissues of corpus cavernosum were taken and RT-PCR was used to detect the mRNA expression of nNOS, iNOS and eNOS in CC, and Western-blot was used to detect the protein expression ofthese NOS isoforms. Results The ICP in the group B was significantly decreased compared to the group A (P<0.01), but the ICP values in the groups C and D were both increased compared to those in the group B (both P <0. 01 ). The expressions of the mRNA and protein of nNOS, iNOS, and eNOS were all decreasedin the group B, however, the mRNA and protein expressions of eNOS were increased a in the groups C and D. In the group C, iNOS also increased. The expression of nNOS showed no obvious changes in the group C and group D.Conclusion Chronic oral treatment with Icariin increases the erectile function(ICP)and restores the eNOS expression in CC of A-ED rats.Icariin may have a long-trem therapeutic effect on ischemia/hypoxia induced ED.

关 键 词：淫羊藿苷 动脉性ED模型 大鼠 阴茎海绵体压力 一氧化氮合酶亚型 NOS 亚型表达 阳萎 勃起功能 

分 类 号：R285[医药卫生—中药学]