血小板反应素mRNA表达、血浆PF4水平与甲状腺乳头状癌颈部淋巴结转移的关系  

Relationship between Expression of TSP mRNA,Concentration of Plasma PF4 and Cervical Lymph Node Metastasis in Papillary Thyroid Carcinoma

作　　者：连云宗[1] 陈一峰[1] 庄建良[1] 

机构地区：[1]福建医科大学附属泉州第一医院,福建泉州362000

出　　处：《中国病毒病杂志》2008年第6期427-430,共4页Chinese Journal of Viral Diseases

基　　金：泉州市科技计划项目(2004Z25)

摘　　要：目的探讨血小板反应素(TSP)mRNA表达、血浆血小板第4因子(PF4)水平与甲状腺乳头状癌(PTC)颈部淋巴结转移的关系。方法应用逆转录多聚酶链反应(RT-PCR)技术检测40例PTC癌组织和10例癌旁正常组织TSP-1、TSP-2 mRNA的表达,并用酶标记免疫分析法(ELISA)测定40例PTC患者、20例健康体检者(对照组)的血浆PF4水平。结果(1)PTC癌组织TSP-1 mRNA、TSP-2 mRNA阳性表达率分别为45.0%、47.5%,它们均明显低于正常组织(80.0%)(P<0.01),而且TSP-1 mRNA、TSP-2 mRAN阳性表达PTC组的颈部淋巴结转移率均明显低于TSP-1 mRNA、TSP-2 mRNA阴性表达PTC组(33.3% vs 90.9%、42.1% vs 85.7%,P<0.01)。(2)正常对照组、无淋巴结转移的PTC组和伴淋巴结转移的PTC组间的PF4血浆浓度呈线性递减趋势,其中无淋巴结转移组的PF4血浆浓度(2.96±0.21)μg/L高于转移组(0.98±0.17)μg/L(P<0.05)。(3)TSP-1 mRNA、TSP-2 mRNA阳性表达PTC组的血浆PF4水平分别明显高于TSP-1 mRNA、TSP-2 mRNA阴性表达组[(2.36±0.91)μg/L vs(1.11±0.60)μg/L,(2.15±1.00)μg/L vs(1.23±0.72)μg/L](P<0.01)。结论缺乏TSP-1 mRNA、TSP-2 mRNA表达及血浆低水平的PF4诸因素可能为促进PTC发生颈部淋巴结转移起到了推波助澜的作用,TSP-1、TSP-2与PF4在抑制PTC颈部淋巴结转移中起着互相协同的作用。Objective To explore the relationship between expression of thrombospondin (TSP) mRNA,concentration of plasma PF4 and cervical lymph node metastasis in papillary thyroid carcinoma (PTC).Methods RT-PCR technique was utilized to detect the expression of TSP-1 and TSP-2 mRNA in 40 cases of PTC and 10 eases of nomal tissue surrounding carci- noma Meanwhile enzyme-linked immunosorbant assay (ELISA) technique was used to measure the concentration of human platelet factor 4 (PF4) in plasma of every 40 patients with PTC and 20 healthy persons taken as control Results (1) The positive expression rate of TSP-1 mRNA of PTC was 45%.The positive expression rate of TSP-2 mRNA was 47.5%.The positive expression rate of normal tissue was 80%.Either the positive expression rate of TSP-1 or TSP-2 mRNA of PTC tissues was significantly lower than that of nomal tissues (P<0.01).Furthermore the metastatic rate of cervical lymph node of PTC with TSP-1 mRNA or TSP-2 mRNA positive expression was significantly lower than that of PTC with TSP-1 mRNA or TSP-2 mRNA negative expression accordingly (33.3% vs 90.9%,42.1% vs 85.7% ) (P<0.01).(2) The concentration of PF4 in plasma of normal healthy person,PTC with cervical lymph node metastasis and PTC without cervical lymph node metastasis was in linear progressive decreasing tendency.The plasma concentration of PF4 of PTC without cervical lymph node metastasis [(2.96±0.21)μg/L] was higher than that of PTC with cervical lymph node metastasis [(0.98±0.17)μg/L] (P<0.05). (3) The plasma concentration of PF4 of PTC with TSP-1 mRNA or TSP-2 mRNA positive expression was higher than that of PTC with TSP-1 mRNA or TSP-2 mRNA negative expression [(2.36±0.91)μg/L vs (1.11±0.60)μg/L,(2.15±1.00)μg/L vs (1.23±~0.72)μg/L (P<0.01)].Conclusion Deficiency of TSP-1 mRNA or TSP-2 mRNA expression and low plasma level of PF4 may become such promoters as fuel added to the flames in the process of cervical lymph node metas- tasis of PTC.TSP-1,TSP-2 and PF4 serve as coordinate factors in the inhibition of ce

关 键 词：甲状腺肿瘤 TSP PF4 RNA 信使 淋巴结转移 

分 类 号：R736.1[医药卫生—肿瘤]