腹膜腔注射肝素对ACC-M细胞在裸鼠肺、肝组织分布的影响  

The Effect of Intraperitoneal Heparin on Distribution of ACC-M in Lung and Liver

作　　者：王新军[1] 陈万涛[2] 周正炎[1] 马衍青[3] 周晓健[2] 

机构地区：[1]同济大学附属同济医院口腔颌面外科,上海200065 [2]上海第二医科大学附属第九人民医院口腔颌面外科,上海200065 [3]中国科学院上海细胞所,上海200065

出　　处：《华西口腔医学杂志》2004年第3期246-247,251,共3页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目 (39970 794)

摘　　要：目的　通过观察腹膜腔注射肝素对ACC_M细胞在裸鼠肺、肝组织的分布特点 ,研究其对腺样囊性癌肺高转移细胞肺粘附的抑制作用。方法　实验裸鼠腹腔注射肝素后 ,经尾静脉注入氚标胸腺嘧啶核苷 (3 HTdR)标记ACC_M细胞 ,分别检测注射后 2h、6h、18h肺、肝组织单位重量的每分钟同位素脉冲数 (CPM)。结果　裸鼠单位重量肺组织3 H_ACC_M在不同时间由高到低依次是空白对照组 2h、6h、18h ,肝素组 6h、2h、18h。相同时段肝素组明显低于对照组 ,两组间有显著性差异 (P <0 0 0 1)。单位重量肝组织3 H_ACC_M在不同时间由高到低依次是空白对照组为 2h、6h、18h ,肝素组为 2h、6h、18h ,相同时段两组间无显著性差异 (P >0 0 5 )。结论　肝素腹腔注射后可减少肺组织内ACC_M细胞数量 ,但对相同时段肝组织内ACC_M细胞数量无显著影响 ,肝素可能在抑制腺样囊性癌肺转移中发挥作用。Objective To investigate the effect of intraperitoneal heparin on the adhesion of highly lung metastasic adenoid cystic carcinoma cell line (ACC-M) in lung.Methods +3HTdR labeled ACC-M cells were injected by intravenous infusion after intraperitoneal injection with 200 units heparin. 4 mice of each group were killed at 2 h, 6 h and 18 h after infusion. The relative radioactivity (CPM) in lung and liver was detected.Results +3H-activity per gram in lung of heparin group was lower than control at the same time. There was significant difference between the two groups (P<0.001). There was no difference between the two groups in liver (P>0.05).Conclusion The results of this study suggest that the highly lung metastasis characteristic of ACC-M may be partially inhabited by the use of intraperitoneal heparin.

关 键 词：肝素 ACC-M细胞 粘附 

分 类 号：R739.8[医药卫生—肿瘤]