重组人骨形成蛋白-2促进兔下颌牵张成骨的研究  

Recombinant Human BMP-2 Accelerates Bone Formation of Mandibular Distraction Osteogenesis in Rabbits

作　　者：王志国[1] 胡静[1] 邹淑娟[1] 李继华[1] 高占巍[1] 王大章[1] 

机构地区：[1]口腔生物医学工程教育部重点实验室,四川大学,四川成都610041

出　　处：《华西口腔医学杂志》2004年第3期186-188,共3页West China Journal of Stomatology

基　　金：教育部跨世纪优秀人才基金资助 (教技函 2 0 0 2_48)

摘　　要：目的　研究局部应用基因重组人骨形成蛋白_2 (rhBMP_2 )对兔下颌牵张成骨的影响。方法　在 12只成年大耳白兔的双侧下颌骨前部行骨切开术 ,将rhBMP_2与胶原复合植入一侧下颌骨切开处 ,另一侧单纯植入胶原作对照。用自行研制牵张器延长双侧下颌骨 6mm ,在牵张结束后第 4周处死动物 ,取双侧牵张区新生骨痂行组织学、扫描电镜及Ca/P元素测定。结果　下颌延长后两侧牵张间隙均有新骨形成 ,应用rhBMP_2的一侧牵张骨痂中的新骨组织比对照侧多而成熟 ,钙化程度较高。结论　基因重组人骨形成蛋白_2可能有促进兔下颌牵张成骨的作用。Objective To study the effects of rhBMP-2 on bone formation of mandibular distraction osteogenesis in rabbits.Methods Bilateral mandibular osteotomies were performed in 12 mature rabbits. 5 mg rhBMP-2 with the collagen carrier was implanted in the osteotomy site of one side of the mandibles. Only the collagen sponge was placed in the contra-lateral side as control. The mandibles of 8 rabbits were lengthened by 6mm using a custom-made distractor. At 4 weeks after the end of distraction, all animals were killed and the distracted calluses were harvested and processed for histological, scanning electron microscopic, as well as Ca/P ratio analysis.Results The regenerated bone was found in the distraction gap after mandibular lengthening. The mandibular side treated with rhBMP-2 had greater amounts of new bone formation and earlier mineralization than contra-lateral side (non-rhBMP-2 treated).Conclusion Recombinant human BMP-2 appears to be able to accelerate bone formation of mandibular distraction osteogenesis in rabbits.

关 键 词：下颌骨牵张 重组人骨形成蛋白-2 新骨生成 

分 类 号：R782.2[医药卫生—口腔医学]