Production of β-1,3-glucanase and chitinase of two biocon-trol agents and their possible modes of action  

Production of p-1,3-glucanase and chitinase of two biocon-trol agents and their possible modes of action

作　　者：FAN Qing, TIAN Shiping, LIU Haibo & XU YongInstitute of Botany, Chinese Academy of Sciences, Beijing 100093, China 

出　　处：《Chinese Science Bulletin》2002年第4期292-296,共5页

基　　金：This work was supported by the National Natural Science Foundation of China (Grant No. 30170663) ; Young Scientists Creative Group for Life Science and Biological Technology of the Chinese Academy of Sciences.

摘　　要：Pichia membranefaciens Hansen and Candida guilliermondii (Cast) Langeronet Guerra are two antagonists of R. stolonifer on harvested nectarine and peach fruits. In this study, β-1,3-glucanase and chitinase activities of the antagonists were induced in vitro and in vivo. The highest β-1, 3-glucanase activity was detected in Lilly-Barnett minimal salt medium supplemented with glucose in combination with CWP of R. stolonifer as a carbon source. The β-1,3-glucanase activity of P. membranefaciens reached the maximum level, being 114.0 SU (specific activity unit), and that of C. guilliermondii reached 103.2 SU. The lowest β-1,3-glucanase activity was observed in the medium containing glucose as sole car-bon source. P. membranefaciens was able to produce signifi-cantly higher levels of chitinase (exochitinase and endochiti-nase) in vitro than C. guilliermondii grown in Czapeck mini-mal medium. An increase in β-1,3-glucanase and chitinase activity was also triggered by wounding, adding of carbon sourcesPichia membranefaciens Hansen and Candida guilliermondii (Cast) Langeronet Guerra are two antagonists of R. stolonifer on harvested nectarine and peach fruits. In this study, β-1,3-glucanase and chitinase activities of the antagonists were induced in vitro and in vivo. The highest β-1, 3-glucanase activity was detected in Lilly-Barnett minimal salt medium supplemented with glucose in combination with CWP of R. stolonifer as a carbon source. The β-1,3-glucanase activity of P. membranefaciens reached the maximum level, being 114.0 SU (specific activity unit), and that of C. guilliermondii reached 103.2 SU. The lowest β-1,3-glucanase activity was observed in the medium containing glucose as sole car-bon source. P. membranefaciens was able to produce signifi-cantly higher levels of chitinase (exochitinase and endochiti-nase) in vitro than C. guilliermondii grown in Czapeck mini-mal medium. An increase in β-1,3-glucanase and chitinase activity was also triggered by wounding, adding of carbon sources and yeast cells. The results showed that both β-1,3-glucanase and chitinase from P. membranefaciens and C. guilliermondii exhibited some effects on controlling R. stolonifer, and might have a synergistic activity against R. stolonifer.

关 键 词：biological control YEAST RHIZOPUS ROT β-1 3-GLUCANASE chitinase. 

分 类 号：Q55[生物学—生物化学]