Clonogenic colony-forming ability of hepatic stem cells in the spleens of mice  

作　　者：SU Juan YAO Yucheng YU Hongyu ZHU Haiyinq WANG Xinmin WANG Zhonghua HU Yiping 

机构地区：[1]Department of Cell Biology,the Second Military Medical University,Shanghai 200433,China [2]Department of Pathology,the Second Military Medical University,Shanghai 200433,China

出　　处：《Chinese Science Bulletin》2002年第3期211-213,共3页

基　　金：This work was supported bythe program issued by the Ministry of Science and Technology of China (Grant No. TJ99-LA01) ; the Program for Backbone College Teachers issued by the Ministry of Education of China.

摘　　要：To confirm the existence of hepatic stem cells (HSCs), fetal liver cells isolated from mice on embryonic day 13 (ED13) were long-term cultured in vitro. Growth of the cells was observed intensively and characteristics were iden-tified by immunocytochemistry. The results showed that some of the cells grew as colonies, in which some cells ex-pressed AFP, CD34 and Albumin. Then the cells were trans-planted intravenously into irradiated syngeneic mice. At day 12 a number of small hyperplasia nodules were seen in the apparently enlarged spleens of recipient mice. Moreover, some nodules were positive for AFP and CD34 and consisted of various types of cells, suggesting the very existence of he-patic stem cells in the mouse fetal liver.To confirm the existence of hepatic stem cells (HSCs), fetal liver cells isolated from mice on embryonic day 13 (ED13) were long-term cultured in vitro. Growth of the cells was observed intensively and characteristics were identified by immunocytochemistry. The results showed that some of the cells grew as colonies, in which some cells expressed AFP, CD34 and Albumin. Then the cells were transplanted intravenously into irradiated syngeneic mice. At day 12 a number of small hyperplasia nodules were seen in the apparently enlarged spleens of recipient mice. Moreover, some nodules were positive for AFP and CD34 and consisted of various types of cells, suggesting the very existence of hepatic stem cells in the mouse fetal liver.

关 键 词：hepatic stem CELLS fetal liver CELLS in vivo transplantation colony-forming-units CLONAL growth hepatogenous SPLENIC hyper-plasia nodules. 

分 类 号：Q785[生物学—分子生物学]