Identification of a novel HMW glutenin subunit and comparison of its amino acid sequence with those of homologous subunits  

作　　者：YAN Zehong WAN Yongfang LIU Kunfan ZHENG Youliang WANG Daowen 

机构地区：[1]State Key Laboratory of Plant Cell and Chromosome Engineering,Institute of Genetics,Chinese Academy of Sciences,Beijing 100101,China [2]Triticeae Research Institute,Sichua Agriculture University,Dujiangyan 611830,China

出　　处：《Chinese Science Bulletin》2002年第3期220-225,共6页

基　　金：This work was supported by the "973" Key Basic Research and Development Program (Grant No. G1998010208) ; the special fund for biotechnology research from the Chinese Academy of Sciences.

摘　　要：Aegilops tauschii is the donor of the D genome of common wheat (Triticum aestivum). Genetic variation of HMW glutenin subunits encoded by the Glu-1Dt locus of Ae. tauschii has been found to be higher than that specified by the Glu-1D locus in common wheat. In the present note, we report the identification of a novel HMW glutenin subunit, Dy13t, from Ae. tauschii. The newly identified subunit pos-sessed an electrophoretic mobility that was faster than that of the Dy12 subunit of common wheat. The complete ORF of encoding the Dy13t subunit contained 624 codons (excluding the stop codons). The amino acid sequence deduced from the Dy13t gene ORF was the shortest among those of the previ-ously reported subunits derived by the D genome. A further comparison of Dy13t amino acid sequence with those of the subunits characterized from the A, B, D, R genomes of Triticeae showed that the smaller size of the Dy13t subunit was associated with a reduction in the size of its repetitive domain.Aegilops tauschii is the donor of the D genome of common wheat (Triticum aestivum). Genetic variation of HMW glutenin subunits encoded by the Glu-Ud(t)locus of Ae. tauschii has been found to be higher than that specified by the Glu-1D locus in common wheat. In the present note, we report the identification of a novel HMW glutenin subunit, Dy13(t), from Ae. tauschii. The newly identified subunit possessed an electrophoretic mobility that was faster than that of the Dy12 subunit of common wheat. The complete ORF of encoding the Dy13(t) subunit contained 624 codons (excluding the stop codons). The amino acid sequence deduced from the Dy13(t) gene ORF was the shortest among those of the previously reported subunits derived by the D genome. A further comparison of Dy13(t) amino acid sequence with those of the subunits characterized from the A, B, D, R genomes of Triticeae showed that the smaller size of the Dy13(t) subunit was associated with a reduction in the size of its repetitive domain.

关 键 词：WHEAT Ae. tauschii HMW GLUTENIN subunlt. 

分 类 号：Q946[生物学—植物学]