Bioinformatic analysis of human nuclear receptor nr5a2(hb1f) genomic sequence  

作　　者：Zhang Chengkang Lin Wei Cai Yanning Dong Hui Li Mei Fu Gang Xie Youhua Huang M.Guyang Wang Yuan 

机构地区：[1]Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,200031,Shanghai,China [2]Chinese Human Genome Center at Shanghai,201203,Shanghai,China

出　　处：《Chinese Science Bulletin》2002年第7期568-572,617,共6页

基　　金：This work was supported by the National Natural Science Foundation of China (Grant No. 39893320-4);the Chinese Human Genome Center at Shanghai (Grant No. CNCS-98-M-06);the Basic Research Program from the Ministry of Science ; Technology (Grant No. G19

摘　　要：We have cloned the cDNA of human nuclear receptor nr5a2(hb1f) gene and obtained its whole genomic sequence previously. In this work we carried out in-depth bioinformatic analysis on the genomic sequence of nr5a2(hb1f) gene. Sequence comparison and prediction algorithms implicated that there might be additional coding regions in the 210 kb genomic sequence besides known exons, especially in the two largest introns. Comparison of the structures of nr5a loci in different species revealed distinguishable conservation and apparent gene duplication during evolution. The remarkable conservation among promoters of zebrafish, mouse and human nr5a2 genes suggested that they would be regulated by the same transcription factors.We have cloned the cDNA of human nuclear receptor nr5a2(hb1f) gene and obtained its whole genomic sequence previously. In this work we carried out in-depth bioinformatic analysis on the genomic sequence of nr5a2(hb1f) gene. Sequence comparison and prediction algorithms implicated that there might be additional coding regions in the 210 kb genomic sequence besides known exons, especially in the two largest introns. Comparison of the structures of nr5a loci in different species revealed distinguishable conservation and apparent gene duplication during evolution. The remarkable conservation among promoters of zebrafish, mouse and human nr5a2 genes suggested that they would be regulated by the same transcription factors

关 键 词：NUCLEAR RECEPTOR nr5a2(hb1f) GENE structure bioinfor-matics COMPARATIVE genomics. 

分 类 号：Q987[生物学—遗传学]