Isolation of tomato proteinase inhibitor Ⅱ gene and the function of its intron  

Isolation of tomato proteinase inhibitor II gene and the function of its intron

作　　者：Xie, XZ Wu, NH 

机构地区：[1]Chinese Acad Sci, Inst Dev Biol, Beijing 100080, Peoples R China

出　　处：《Chinese Science Bulletin》2002年第10期830-834,共5页

基　　金：This work was supported by the Langqidao Company, Fuzhou Municipality, Fujian Province; and the State Tobacco Monopoly Administration.

摘　　要：The genomic DNA sequence of tomato pro-teinase inhibitor II gene (named tin2i, whose accession number in GenBank is AF007240) was isolated by PCR techniques. The intron sequence (TPI), with a length of 109 bp, owns typical structures of GT/AG dinucieotides at both ends and high content of AT base pairs which accounts for 80.7% of the total nucleotides. As shown by recombination experiment, the TPI sequence could efficiently promote the expression of the reporter gene gusA and this effect was independent of the position and orientation of the intron, thus showing its role as an enhancer. Such experiments as gel retardation assays, GUS histochemical staining and GUS fluorometric assays further demonstrated that TPI sequence maybe has promoter-like activity.The genomic DNA sequence of tomato pro-teinase inhibitor II gene (named tin2i, whose accession number in GenBank is AF007240) was isolated by PCR techniques. The intron sequence (TPI), with a length of 109 bp, owns typical structures of GT/AG dinucieotides at both ends and high content of AT base pairs which accounts for 80.7% of the total nucleotides. As shown by recombination experiment, the TPI sequence could efficiently promote the expression of the reporter gene gusA and this effect was independent of the position and orientation of the intron, thus showing its role as an enhancer. Such experiments as gel retardation assays, GUS histochemical staining and GUS fluorometric assays further demonstrated that TPI sequence maybe has promoter-like activity.

关 键 词：TOMATO PROTEINASE INHIBITOR II GENE INTRON enhancer. 

分 类 号：Q943[生物学—植物学]