Expression of hepatitis B surface antigen gene (HBsAg) in Laminaria japonica (Laminariales,Phaeophyta)  

作　　者：JIANG Peng QIN Song TSENG Chengkui 

机构地区：[1]Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China [2]Graduate School,Chinese Academy of Sciences,Beijing 100039,China

出　　处：《Chinese Science Bulletin》2002年第17期1438-1440,共3页

基　　金：This work was supported by National Climbing Plan B(Grant No. PDB6-4-1);National Natural Science Foundation of China (Grant No. 39400076);and Shandong Province Gongguan Plan (Grant No. 991051109).

摘　　要：A transformation model for Laminaria japonica was established from 1993 to 1998, on the basis of which the transgenic kelp with heterologous gene encoding hepatitis B surface antigen (HBsAg) was obtained by using the micro-particle bombardment transformation method. Results of quantitative ELISA showed that HBsAg in transgenic kelp was 0.529μg/mg soluble proteins on average and the highest value was 2.497μg/mg, implying that recombinant HBsAg had natural epitope. Further support for the integration of HBsAg gene into kelp genome was obtained by PCR-Southern and total DNA hybridization. Prospect of kelp bio-reactor producing high value materials such as edible HBY vaccine was discussed as well.A transformation model for Laminaria japonica was established from 1993 to 1998, on the basis of which the transgenic kelp with heterologous gene encoding hepatitis B surface antigen (HBsAg) was obtained by using the microparticle bombardment transformation method. Results of quantitative ELISA showed that HBsAg in transgenic kelp was 0.529 μg/mg soluble proteins on average and the highest value was 2.497 μg/mg, implying that recombinant HBsAg had natural epitope. Further support for the integration of HBsAg gene into kelp genome was obtained by PCR-Southern and total DNA hybridization. Prospect of kelp bioreactor producing high value materials such as edible HBV vaccine was discussed as well.

关 键 词：LAMINARIA japonica genetic engineering hepatitis B surface antigen (HBsAg) EXPRESSION system vaccine. 

分 类 号：R373.2[医药卫生—病原生物学]