Expressing PHB synthetic genes through chloroplast genetic engineering  

作　　者：ZHANG Jingyu SU Ning ZHANG Zhonglin ZHAO Huayan ZHU Shengwei SONG Yanru 

机构地区：[1]Key Laboratory of Photosynthesis and Enviromental Molecular Physiology,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China [2]Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China

出　　处：《Chinese Science Bulletin》2002年第16期1373-1376,共4页

基　　金：This work was supported by the State "863" High-Tech Program (Grant No. 101-04-03-04);the National Natural Science Foundation of China (Grant No. 59673008).

摘　　要：Chloroplast integration and expression vector containing expression cassettes for phbB, phbA, phbC and aadA genes was constructed and bombarded into the tobacco chloroplast genome. Transplastomic plants were analyzed with PCR and Southern blot. Their homoplastomy was also judged. Northern dot and RT-PCR analysis were employed to investigate transgene expression at transcriptional level. The results indicate that the chloroplast transformation system is compatible for poly-3-hydroxybutyrate (PHB) production.Chloroplast integration and expression vector containing expression cassettes for phbB, phbA, phbC and aadA genes was constructed and bombarded into the tobacco chloroplast genome. Transplastomic plants were analyzed with PCR and Southern blot. Their homoplastomy was also judged. Northern dot and RT-PCR analysis were employed to investigate transgene expression at transcriptional level. The results indicate that the chloroplast transformation system is compatible for poly-3-hydroxybutyrate (PHB) production.

关 键 词：CHLOROPLAST genetic engineering poly-3-hydroxybutyrate transplastomic tobacco TRANSCRIPTIONAL level gene silencing. 

分 类 号：Q943.2[生物学—植物学]