Molecular cloning, identification and characteristics of NYD-SP9:Gene coding protein kinase presumably involved in spermatogenesis  

作　　者：XIAO Junhua YIN Lanlan LI Jianmin ZU Hu ZHOU Zuomin ZHAO Baige SHA Jiahao 

机构地区：[1]Lab of Reproductive Medicine,Nanjing Medical University,Nanjing 210029,China [2]Department of International Cooperation,State Family Planning Commission,Beijing 100088,China

出　　处：《Chinese Science Bulletin》2002年第11期896-901,共6页

基　　金：This work was supported by the State "973" Fund (Grant No. G1999055901).

摘　　要：Using cDNA microarray hybridization from a human testicular cDNA library, one gene exhibiting ten-fold difference at expression level between adult and embryo human testes was cloned and named NYD-SP9, which was believed to be involved in spermatogenesis. Southern blot hybridization results showed that NYD-SP9 expressed highly in testis but low in ovary. Protein motif analysis of this cDNA sequence revealed a cluster of phosphorylation sites, indicating its potential involvement in signal pathways during spermatogenesis. Furthermore, one transmembrane helix was predicted in N-terminal region, indicating that putative NYD-SP6 may be served as a transmembrane protein. The proximity of these potential phosphorylation sites to each other indicates that there may be interaction among these sites to regulate spermatogenesis. These findings suggested that protein kinase NYD-SP9 might play a role in male germ cell differentiation.Using cDNA microarray hybridization from a human testicular cDNA library, one gene exhibiting ten-fold difference at expression level between adult and embryo human testes was cloned and named NYD-SP9, which was believed to be involved in spermatogenesis. Southern blot hybridization results showed that NYD-SP9 expressed highly in testis but low in ovary. Protein motif analysis of this cDNA sequence revealed a cluster of phosphorylation sites, indicating its potential involvement in signal pathways during spermatogenesis. Furthermore, one transmembrane helix was predicted in N-terminal region, indicating that putative NYD-SP6 may be served as a transmembrane protein. The proximity of these potential phosphorylation sites to each other indicates that there may be interaction among these sites to regulate spermatogenesis. These findings suggested that protein kinase NYD-SP9 might play a role in male germ cell differentiation.

关 键 词：differential expression gene human TESTIS NYD-SP9. 

分 类 号：Q55[生物学—生物化学]