DNA microarray synthesis by using PDMS molecular stamps (Ⅲ)——Optimization for the reaction conditions  

作　　者：XIAO Pengfeng, LU Zuhong, LIU Zhengchun, HE Quanguo & HE NongyueKey Laboratory for Molecular and Biomolecular Electronics of Ministry of Education, Southeast University, Nanjing 210096, China 

出　　处：《Chinese Science Bulletin》2002年第13期1073-1076,共4页

基　　金：This work was supported by the 863 National High Technology Research ; Development Program (Grant Nos. 103-13-05-01 and 101-01-01);the 973 National Key Fundamental Research Project (Grant No. G1998051204);the National Science Foundation of China (Gra

摘　　要：Optimization for the technological processes of fabricating oligonucleotide microarray by the molecular stamping method is studied in this note. Three factors that affect the pressing coupling reactions of the nucleosides are focused on: the stability of the chemical activities of the reaction solutions, the contamination of the remain of the reactive nucleotides among the different spots on the chip, and the influence of the capping reaction on the hybridization result. The experiments show that the acetonitrile solution of tetrazole and nucleoside monomer could maintain sufficient reactive activity for more than 10 h. An effective method has been used and proved to eliminate the residual reactive nucleosides on chip with small molecules containing hydroxylgroup. Finally, the capping step-a regular step in theconventional DNA chemical synthesis can be neglected in our on-chip DNA synthetic process, which would not affect its hybridization results.Optimization for the technological processes of fabricating oligonucleotide microarray by the molecular stamping method is studied in this note. Three factors that affect the pressing coupling reactions of the nucleosides are focused on: the stability of the chemical activities of the reaction solutions, the contamination of the remain of the reactive nucleotides among the different spots on the chip, and the influence of the capping reaction on the hybridization result. The experiments show that the acetonitrile solution of tetrazole and nucleoside monomer could maintain sufficient reactive activity for more than 10 h. An effective method has been used and proved to eliminate the residual reactive nucleosides on chip with small molecules containing hydroxylgroup. Finally, the capping step-a regular step in theconventional DNA chemical synthesis can be neglected in our on-chip DNA synthetic process, which would not affect its hybridization results.

关 键 词：OLIGONUCLEOTIDE MICROARRAY MOLECULAR STAMPING method DNA chemical synthesis. 

分 类 号：Q523[生物学—生物化学]