Cloning and expression of the Chinese wheat mosaic virus RNA2 coat protein read-through and 19 ku cysteine-rich domains and localization of these proteins  

作　　者：XU Lei CHEN Jianping YE Rong ZHAO Xiuyun YU Shanqing 

机构地区：[1]Microbiology Department,Fudan University,Shanghai 200433,China [2]Virology Laboratory,Zhejiang Academy of Agricultural Sciences,Hangzhou 200433,China

出　　处：《Chinese Science Bulletin》2002年第12期1019-1023,共5页

基　　金：This work was supported by the Zhejiang Provincial Natural Science Foundation (Grant Nos. 399421 and RC9604);the National Natural Science Foundation of China (Grant No. 399704820);a special grant for the Key Laboratory of Zhejiang Province (Grant No. 0

摘　　要：The 5’-terminal (RTn) and 3’-terminal (RTc) halves of the coat protein readthrough domain and the 19 ku cysteine-rich protein of Chinese wheat mosaic virus (CWMV) were amplified by RT-PCR, cloned and expressed in E. coll. Antisera and monoclonal antibodies against these proteins were prepared by immunising these purified proteins to mice. Detection of RTn, RTc and 19 ku proteins in CWMV infected wheat sap and leaf tissue indicated that the RTn and RTc proteins were distributed on the surface of virus particles whereas the 19 ku protein was in the cytoplasm of the infected wheat cells.The 5′-terminal (RTn) and 3′-terminal (RTc) halves of the coat protein readthrough domain and the 19 ku cysteine-rich protein of Chinese wheat mosaic virus (CWMV) were amplified by RT-PCR, cloned and expressed inE. coli. Antisera and monoclonal antibodies against these proteins were prepared by immunising these purified proteins to mice. Detection of RTn, RTc and 19 ku proteins in CWMV infected wheat sap and leaf tissue indicated that the RTn and RTc proteins were distributed on the surface of virus particles whereas the 19 ku protein was in the cytoplasm of the infected wheat cells.

关 键 词：CHINESE WHEAT mosaic virus coat protein READTHROUGH DOMAIN CYSTEINE-RICH DOMAIN prokaryotic expression localisation. 

分 类 号：Q78[生物学—分子生物学]