Gene cloning and function analysis of ABP9 protein which specifically binds to ABRE2 motif of maize Cat1 gene  

作　　者：Lei Wang Jun Zhao Yunliu Fan 

机构地区：[1]Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China

出　　处：《Chinese Science Bulletin》2002年第22期1871-1875,共5页

基　　金：This work was supported by the National Key Basic Research Program (Grant No. G1999011703);the National Trans-genie Plants Research and Commercialization Program (Grant No. J99-A-028).

摘　　要：A cDNA library was constructed using mRNA extracted from 17 days post-pollination (dpp) maize embryos and was screened by employing a yeast one-hybrid system for proteins specifically interacting with ABRE2 motif of maize Call gene. Three truncated overlapping positive clones designated ABP9 were obtained and the full-length cDNA was isolated by 5’ RACE. Searching the database revealed that ABP9 protein belongs to a bZIP-type transcription factor family. ABP9 protein speciflcally binds to ABRE2 motif and activates the expression of downstream reporter gene in yeast cells. Our results strongly suggest that the ABP9 protein functions as a transcription activator.AcDNA library was constructed using mRNA extracted from 17 days post-pollination (dpp) maize embryos and was screened by employing a yeast one-hybrid system for proteins specifically interacting with ABRE2 motif of maize Cat1 gene. Three truncated overlapping positive clones designated ABP9 were obtained and the full-length cDNA was isolated by 5′ RACE. Searching the database revealed that ABP9 protein belongs to a bZIP-type transcription factor family. ABP9 protein specifically binds to ABRE2 motif and activates the expression of downstream reporter gene in yeast cells. Our results strongly suggest that the ABP9 protein functions as a transcription activator.

关 键 词：MAIZE ABRE ABP TRANSCRIPTION factor function analysis. 

分 类 号：Q785[生物学—分子生物学]