成年大鼠纹状体神经干细胞体外提纯及检测  

Purification and identification of neural stem cells in adult rat's corpus striatum

作　　者：刘振华[1] 王彦惠[1] 刘亚杰[1] 姜晓丹[2] 朱利元[1] 周振军[2] 

机构地区：[1]第一军医大学附属珠江医院神经内科,广东广州510282 [2]第一军医大学附属珠江医院神经外科,广东广州510282

出　　处：《中华神经医学杂志》2002年第1期49-51,共3页Chinese Journal of Neuromedicine

基　　金：军队[01Z054];广东省科技厅[No粤科基办(2000)25];[粤财企(2001)367]重大科技项目基金

摘　　要：目的建立成年大鼠纹状体区神经干细胞的体外提纯方法,并进行纯度检测。方法将成年大鼠的纹状体组织制成单细胞悬液,通过磁性分离系统从细胞悬液中分离出神经干细胞,并以流式细胞仪检测其纯度。结果提纯而来的细胞经干细胞特异性Nestin荧光染色后在荧光显微镜下可见细胞呈圆形且发出绿色荧光,经流式细胞仪检测Nestin荧光染色阳性率87.5 % ±2.5 %。结论利用磁性分离技术可成功将成年大鼠纹状体组织中的神经干细胞提纯。Objective To creat the method of purificating the neural stem cells from adult ratcorpus striatum in vitro and detect the purity of the sorted cells. Methods Suspension of adult rat corpus striatum cells was incubated with anti-nestin antibody , and the labeled cells were separated form the cell suspension with the magnetic cell sorting system. Purity of the sorted cells was detected with flow cytometer. Results The sorted cells treated with the fluorescence staining were showed green fluorescence under the fluorescence microscope and the purity of the cells was 87.5%±2.5%. Conclusion The magnetic cell sorting system can effectively separate neural stem cells from corpus striatum of adult rat.

关 键 词：神经干细胞 纹状体 免疫磁珠 流式细胞仪 

分 类 号：R741[医药卫生—神经病学与精神病学]