胶质细胞诱导内皮细胞系血脑屏障特点的实验研究  

The influence of astrocyte on immortalized endothelial cell:towards an in vitro blood-brain barrier

作　　者：陈一招[1] 徐如祥[1] 徐宗俊[2] 杨志林[1] 姜晓丹[1] 

机构地区：[1]第一军医大学珠江医院神经外科,广东广州510282 [2]第一军医大学珠江医院检验科,广东广州510282

出　　处：《中华神经医学杂志》2002年第1期62-64,69,共4页Chinese Journal of Neuromedicine

基　　金：国家自然科学基金(3017961);军队十五重点项目基金(01ZD54)

摘　　要：目的探索胶质细胞在血脑屏障形成中的重要意义,建立一套可靠、简便的血脑屏障体外研究方法。方法采用内皮细胞系ECV304与星形胶质细胞体外接触共培养的方法,探索星形胶质细胞对内皮细胞系多种血脑屏障特点的诱导作用。结果星形胶质细胞可以诱导内皮细胞系形成紧密连接并产生较高的跨内皮阻抗(transendothelial electricalresistance, TER),同时,内皮细胞系的血脑屏障特征酶γ-谷氨酰转肽酶(γ-glutamyl transpeptidase, γ-GT)与碱性磷酸酶(alkaline phosphatase , ALP)活性明显增强。结论星形胶质细胞可以诱导ECV304细胞产生紧密连接等血脑屏障特点,ECV304细胞与星形胶质细胞的体外共培养可以作为研究血脑屏障结构与功能的一种可靠而简便的体外实验方法。Objective To investigate the influences of astrocytes on endothelial cell line ECV304 and to establish a convenient and reliable method for research of blood-brain barrier in vitro. Methods After coculturing of ECV304 with astrocytes, transendothelial electrical resistance(TER) of monolayer of ECV304 was determined and the presence of tight junctions between endothelial cells was investigated by electrical microscope. The activity of γ-glutamyl transpeptidase (γ-GT) and alkaline phosphatase (ALP) were measured. Results Astrocytes could induce tight junctions between endothelial cells and increase the TER of monolayer of ECV304.The activity of γ-GT and ALP of ECV304 were increased after coculture with astrocyte. Conclusion Some structural and functional characteristics of blood-brain barrier can be induced in ECV304 by astrocytes. The method of coculture between ECV304 and astrocytes can be considered as a useful model for research of blood-brain barrier in vitro.

关 键 词：胶质细胞 内皮细胞 血脑屏障 跨内皮阻抗 

分 类 号：R741[医药卫生—神经病学与精神病学]