水牛与黄牛卵母细胞的回收及体外成熟、体外受精比较  

COMPARING OF OOCYTES RECOVERY?IVM AND IVF BETWEEN CATTLE AND BUFFALO

作　　者：王启胜[1] 牛树理[2] 

机构地区：[1]上海市畜牧兽医站,上海201103 [2]南京农业大学动物科技学院,江苏南京210095

出　　处：《草食家畜》2001年第S2期141-143,156,共4页Grass-Feeding Livestock

摘　　要：本试验平均从每头水牛的卵巢上获得 4.58个卵母细胞 ,其中可用卵为 2 .97个 ,占 6 5% ,卵母细胞在数量和质量上都低于黄牛 ,差异极显著 (P <0 .0 1 )。黄牛卵母细胞体外成熟、受精率显著高于水牛 (P <0 .0 5)The study was designed to evaluate the effects of area on ovarian follicle population and oocytes quality in buffalo and cattle. Results indicated that Hennan's buffalo had lower number population and poor quality compared to Anhui's buffalo (p<0.05),the oocyte of buffalo was lower number population and poor quality than cattle (p<0.01). And this study was to elucidate the possibility of applying bovine IVF procedure on buffalo IVF. Aspiration of 379 buffalo ovaries has provided an average of 4.58 oocytes per ovary, which is about 1/2 obtained from bovine ovary. The maturation rate (60%) and cleavage rate (25.1%) of buffalo oocytes were significantly lower (p<0.01) than those of cattle oocytes (72%,41.6%).

关 键 词：水牛 卵母细胞 体外回收 体外成熟 

分 类 号：S823[农业科学—畜牧学] S852.1[农业科学—畜牧兽医]