用基因枪法转bar基因以培育抗除草剂的直播稻  

Production of herbicide-resistant direct seedling rice (Oryza Sativa L.) plants from agronomically important japonica varieties via particle bombardment

作　　者：周盛梅[1] 汪晓玲[2] 华志华[2] 薛锐[2] 王慧中[3] 黄纯农[2] 黄大年[2] 

机构地区：[1]浙江大学生命科学院,浙江杭州310028 [2]中国水稻研究所,浙江杭州310006 [3]杭州师范学院生物系,浙江杭州310012

出　　处：《杭州师范大学学报（自然科学版）》2001年第2期20-25,共6页Journal of Hangzhou Normal University(Natural Science Edition)

基　　金：浙江省自然科学资助项目 (3 982 97;3 982 2 7)

摘　　要：用基因枪法将 p CB1 质粒 (含 bar基因和 cecropin B基因 )导入到三种有应用前景的直播稻品种中 ,受体材料为来源于水稻未成熟胚的胚性愈伤组织和来源于成熟胚的悬浮细胞系 .当代转化植株显示出很强的对除草剂 Basta的抗性 ,Southern-blot分析显示 :两个外源基因共整合到转化植株基因组中 ;子一代植株中仍含有外源基因 ;来源于同一块愈伤组织的转化植株的整合模式可能是相似的 ,也可能完全不同 .Particle bombardment has been proved an efficient, variety\|independent DNA transfer fashion. In this report, plasmid pCB\-1\|containing bar and cecropin B gene was transferred into direct seedling rice varieties following particle bombardment. We used embryogenic calli derived from immature embryos and suspension cultures from mature embryos as recipients. The primary transformants show strong basta\|resistance. Southern\|blot analysis demonstrated that gene integration patterns of plants regenerated from embryogenic callus derived from the same explant could be similar, or quite different. Transferred gene inherited in T\-1 plants.\;

关 键 词：BAR基因 除草剂抗性 直播稻 基因枪 胚性愈伤 悬浮培养 

分 类 号：Q813.12[生物学—生物工程]