Induction of Apoptosis in Protoplasts and Suspension Cultures of Plant Cells  

作　　者：颜长辉 周军 陈浩明 戴尧仁 

出　　处：《Tsinghua Science and Technology》2000年第4期428-431,共4页清华大学学报（自然科学版（英文版）

摘　　要：Many studies have showed that apoptosis exists in plants. Our study shows that (1) menadione(VK3) induces apoptosis in suspension cultures of carrot cells; (2) heat shock induces apoptosis in suspension cultures of tobacco cells; and (3) ethrel induces apoptosis in carrot protoplasts. Some important indications of apoptosis were observed, including DNA laddering, TUNEL-positive reaction, condensation and degradation of nuclei.[Many studies have showed that apoptosis exists in plants. Our study shows that (1) menadione(VK3) induces apoptosis in suspension cultures of carrot cells; (2) heat shock induces apoptosis in suspension cultures of tobacco cells; and (3) ethrel induces apoptosis in carrot protoplasts. Some important indications of apoptosis were observed, including DNA laddering, TUNEL-positive reaction, condensation and degradation of nuclei.[

关 键 词：APOPTOSIS PROTOPLASTS suspension culture CARROT tobacco MENADIONE ETHREL heat shock 

分 类 号：Q943.1[生物学—植物学]