Upregulation of drug sensitivity of multidrug resistant SGC7901/VCR human gastric cancer cells by bax gene transduction  

作　　者：赵燕秋 肖冰 陈宝军 乔泰东 樊代明 

机构地区：[1]DepartmentofGastroenterology,XijingHospital,FourthMilitaryMedicalUniversity,Xi’an710032,China [2]DepartmentofGastroenterology,NanfangHospital,FirstMilitaryMedicalUniversity,Guangzhou510515,China

出　　处：《Chinese Medical Journal》2000年第11期17-20,共4页中华医学杂志（英文版）

摘　　要：To investigate the role of bax in a vincristine (VCR) induced multidrug resistant (MDR) human gastric cancer cell line, SGC7901/VCR, in which the Bax protein expression level was significantly lower compared with that in parent cells Methods A bax eukaryotic expression vector was constructed and transfected into SGC7901/VCR cells by lipofectamine, and resistant clones were selected by G418 Western blotting detected Bax expression in transfectants Tetrazolium blue (MTT) assay evaluated the differences in drug sensitivity and cell cycle changes of transfectants were analyzed using flowcytometry (FCM) Results The bax eukaryotic expression vector was constructed and transfected into SGC7901/VCR cells Through G418 selection, resistant clones were obtained Western blotting demonstrated that the expression of Bax protein was markedly increased in bax transduced cells These cells were more sensitive to adriamycin (ADR) and VCR than mock vector transducted cells Moreover, bax transfection enhanced ADR induced apoptosis and VCR induced G 2/M phase arrest of SGC7901/VCR cells Conclusion Bax was involved in the MDR of SGC7901/VCR cellsTo investigate the role of bax in a vincristine (VCR) induced multidrug resistant (MDR) human gastric cancer cell line, SGC7901/VCR, in which the Bax protein expression level was significantly lower compared with that in parent cells Methods A bax eukaryotic expression vector was constructed and transfected into SGC7901/VCR cells by lipofectamine, and resistant clones were selected by G418 Western blotting detected Bax expression in transfectants Tetrazolium blue (MTT) assay evaluated the differences in drug sensitivity and cell cycle changes of transfectants were analyzed using flowcytometry (FCM) Results The bax eukaryotic expression vector was constructed and transfected into SGC7901/VCR cells Through G418 selection, resistant clones were obtained Western blotting demonstrated that the expression of Bax protein was markedly increased in bax transduced cells These cells were more sensitive to adriamycin (ADR) and VCR than mock vector transducted cells Moreover, bax transfection enhanced ADR induced apoptosis and VCR induced G 2/M phase arrest of SGC7901/VCR cells Conclusion Bax was involved in the MDR of SGC7901/VCR cells

关 键 词：bax  · gastric cancer  · multidrug resistance  · gene transduction  · apoptoH 

分 类 号：R735.2[医药卫生—肿瘤]