Selective loss of basal forebrain cholinergic neurons in APP_(770) transgenic mice  

作　　者：李辉 沈孝宙 

机构地区：[1]State Key Laboratory of Reproductive Biology,Ihnstitute of Zoolgy,The Chinese Acadenry of Sciences,Bei jing 100080,China [2]Department of Molecular Biology and Biotechnology,Ihnstitute of Zoolgy,The Chinese Acadenry of Sciences,Bei jing 100080,China

出　　处：《Chinese Medical Journal》2000年第11期80-82,共3页中华医学杂志（英文版）

摘　　要：To determine whether cholinergic neurons in the basal nucleus magnocellularis (NBM) and the medial septum are affected in transgenic mice overexpressing human amyloid precursor protein 770 (APP 770 ) Methods Eight age groups, from 3 months old to 10 months old, of either heterozygous transgenic or non transgenic mice were used for choline acetyltransferase (ChAT) staining using immunohistochemistry The number of ChAT positive neurons was counted on the MCID Image Analysis System Neurons in the cerebral cortex and area CA1 of hippocampus were also stained with cresyl violet and counted using optical dissector technique Results There is no change in the number of forebrain cholinergic neurons in the transgenic mice up to 9 months of age A loss of these cholinergic neurons starts in 9 months old transgenic mice, with a further decrease in the number of NBM and medial septum neurons in 10 month old transgenic mice On the other hand, the number of neurons in the cerebral cortex and hippocampal area CA1 remained unchanged Conclusion These results demonstrate a selective loss of basal forebrain cholinergic neurons in APP 770 transgenic miceTo determine whether cholinergic neurons in the basal nucleus magnocellularis (NBM) and the medial septum are affected in transgenic mice overexpressing human amyloid precursor protein 770 (APP 770 ) Methods Eight age groups, from 3 months old to 10 months old, of either heterozygous transgenic or non transgenic mice were used for choline acetyltransferase (ChAT) staining using immunohistochemistry The number of ChAT positive neurons was counted on the MCID Image Analysis System Neurons in the cerebral cortex and area CA1 of hippocampus were also stained with cresyl violet and counted using optical dissector technique Results There is no change in the number of forebrain cholinergic neurons in the transgenic mice up to 9 months of age A loss of these cholinergic neurons starts in 9 months old transgenic mice, with a further decrease in the number of NBM and medial septum neurons in 10 month old transgenic mice On the other hand, the number of neurons in the cerebral cortex and hippocampal area CA1 remained unchanged Conclusion These results demonstrate a selective loss of basal forebrain cholinergic neurons in APP 770 transgenic mice

关 键 词：Alzheimer's disease amyloid precursor protein CHOLINERGIC neuron loss transgenic model 

分 类 号：R392[医药卫生—免疫学]