Enhanced oxidant stress in synovial vessels of patients on hemodialysis  

作　　者：侯凡凡 王国保 周展眉 张训 

机构地区：[1]Division of Nephrology,Nanfang Hospital

出　　处：《Chinese Medical Journal》2000年第10期70-73,共4页中华医学杂志（英文版）

基　　金：ThisworkwassupportedbyagrantfromtheNationalNaturalScienceFoundationofChina (No 39970 341)

摘　　要：Obejctive To test the hypothesis that the susceptibility of synovial vessels to redox uncoupling which could result in oxidative damage capable of promoting β 2 microglobulin (β 2m) deposition Methods The expression of oxidative markers by microvascular endothelial cells in hemodialysis (HD) patients, non uremic patients and normal subjects was determined using immunostaining and Northern blotting Results Synovial vascular endothelial cells of HD patients exhibited evidence of oxidant stress: induction of malondialdehyde (MDA) epitopes and heme oxygenase These oxidative markers were not detectable in microvascular endothelial cells of other tissues from HD patients, as well as in the synovial vessels of non uremic or normal controls Higher levels of heme oxygenase mRNA in synovia were seen in patients with β 2m deposition compared with patients without β 2m deposition Conclusions Oxidative damage of the synovial microvasculature in uremic patients may be one of the factors which can promote deposition of β 2m in osteo articular tissueObejctive To test the hypothesis that the susceptibility of synovial vessels to redox uncoupling which could result in oxidative damage capable of promoting β 2 microglobulin (β 2m) deposition Methods The expression of oxidative markers by microvascular endothelial cells in hemodialysis (HD) patients, non uremic patients and normal subjects was determined using immunostaining and Northern blotting Results Synovial vascular endothelial cells of HD patients exhibited evidence of oxidant stress: induction of malondialdehyde (MDA) epitopes and heme oxygenase These oxidative markers were not detectable in microvascular endothelial cells of other tissues from HD patients, as well as in the synovial vessels of non uremic or normal controls Higher levels of heme oxygenase mRNA in synovia were seen in patients with β 2m deposition compared with patients without β 2m deposition Conclusions Oxidative damage of the synovial microvasculature in uremic patients may be one of the factors which can promote deposition of β 2m in osteo articular tissue

关 键 词：heme oxygenase MALONDIALDEHYDE ENDOTHELIUM β_2-microglobulin 

分 类 号：R459.5[医药卫生—治疗学]