γ氨基丁酸抑制大鼠海马脑片可溶性淀粉样前体蛋白的释放  

γ-GABA inhibits release of soluble amyloid precursor protein from rat hippocampal brain slices

作　　者：张癸荣[1] 高登莲[2] 曹龙光[3] 殷明[3] 

机构地区：[1]解放军总后勤部卫生部药品仪器检验所,北京100071 [2]解放军第302医院透析科,北京100039 [3]上海交通大学药学院药理学教研室,上海200030

出　　处：《第二军医大学学报》2004年第6期609-611,共3页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(39770807)

摘　　要：目的?观察γ氨基丁酸对可溶性淀粉样前体蛋白(sAPP)释放的影响。方法:雄性SD大鼠断头后取海马于0～4℃ Krebs-Ringer液中作快速振动切片,切片在37℃预孵育后,置于含或不含γ氨基丁酸Krebs-Ringer液中孵育后于15000×g 离心15min取上清,应用二辛可宁酸法测定上清总蛋白含量;释放入孵育液中的sAPP以Western印迹法测定。结果:正常 孵育的海马脑片能够分泌基础量的sAPP,γ氨基丁酸(1.0×10-9～1.0×103μmol/L)可导致海马脑片释放sAPP减少,并且 没有导致新蛋白的产生。结论:γ氨基丁酸在一定浓度范围内能够抑制海马脑片分泌sAPP。Objective:To study the effects of γ-GABA on the release of the soluble amyloid precursor protein(sAPP).Methods:Male Sprague-Dawley rats were decapitated and hippocampus was isolated at4℃,then hippocampal slices were quickly prepared in the medium of Krebs-Ringer buffer at0-4℃by a vibration chopper.After the slices were preincubated in Krebs-Ringer buffer at37℃,they were stimulated with various concentrations of γ-GABA(1.0×10 -9 -1.0×10 3 μmol/L)in Krebs-Ringer buffer.The supernatant was removed from the solution after centrifugated at15000×g.Quantities of total pro-tein were measured by bicinchoninic acid(BCA)assay.The sAPP released into the incubation medium were assayed by West-ern blot.Results:γ-GABA increased sAPP release in the incubated hippocampal brain slices.However,it failed to produce special protein.Conclusion:γ-GABA within certain concentration range has inhibitory effects on the sAPP release.[

关 键 词：Γ氨基丁酸 大鼠 海马脑片 可溶性淀粉样前体蛋白 释放 阿尔茨海默病 

分 类 号：R749.16[医药卫生—神经病学与精神病学]