胰岛素样生长因子在常染色体显性遗传性多囊肾病发病中的作用  

Role of insulin like growth factor -1 in pathogenesis of autosomal dominant polycystic kidney disease

作　　者：吴玉梅[1] 梅长林[1] 孙田美[1] 张维莉[1] 章建全 张玲 宋吉[1] 戴兵[1] 汤兵[1] 

机构地区：[1]第二军医大学长征医院肾内科,上海200003 [2]长征医院超声诊断科

出　　处：《第二军医大学学报》2004年第6期623-625,共3页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(30170901).

摘　　要：目的研究胰岛素样生长因子1(insulin-likegrowthfactor-1,IGF-1)在常染色体显性遗传性多囊肾病(autosomal dominantpolycystickidneydisease,ADPKD)发病中的作用。方法:采用酶联免疫吸附法测定41例ADPKD患者血液、囊肿液 及尿液中IGF-1浓度;应用免疫组织化学染色方法检测IGF-1及其受体(IGF-1R)在ADPKD囊肿组织中的表达。结果:41例 ADPKD患者囊肿液中的IGF-1浓度[(162.0±5.06)ng/ml]显著高于血液[(76.0±28.13)ng/ml]和尿液[(62.0±11.18) ng/ml]中浓度(P<0.01);IGF-1和IGF-1R在ADPKD囊肿组织中的囊肿衬里上皮细胞、平滑肌细胞及间质细胞均呈阳性表 达。结论:囊肿液中IGF-1的增多可能来自囊肿衬里上皮细胞及间质细胞的合成和分泌,并与囊肿的形成和长大有关。Objective:To investigate the role of insulin-like growth factor-1(IGF-1)in the pathogenesis of autosomal dominant polycystic kidney disease(ADPKD).Methods:IGF-1levels in the plasma,urine and cystic fluid from41patients with ADPKD were determined by ELISA.The expressions of IGF-1and IGF-1receptor(IGF-1R)in ADPKD kidney tissues were detected with immunohistochemistry.Results:The levels of IGF-1in cystic fluid [(162.0±5.06)ng/ml]were greatly higher than those in plasma [(76.0±28.13)ng/ml,P<0.01]and urine [(62.0±11.18)ng/ml,P<0.01].IGF-1and IGF-1R were mostly expressed in cystlining-epithelial cells,smooth muscle cells and interstitial cells.Conclusion:The increasing IGF-1in cystic fluid may be from cystlining-epithelial cells and interstitial cells.IGF-1may be involved in the formation and develop-ment of kidney cystis. [

关 键 词：胰岛素样生长因子 常染色体 遗传性多囊肾病 囊肿衬里上皮细胞 

分 类 号：R692[医药卫生—泌尿科学]