CyclinD_1、CDK_4蛋白在食管癌中的表达及意义  

3 Expression and significance of CyclinD 1、CDK 4 in esophageal squamous Cell Carcinoma

作　　者：牛韵韵[1] 高巍[1] 张云汉[1] 晏梅[1] 高冬玲[1] 

机构地区：[1]河南医科大学第一附属医院

出　　处：《中国民康医学》1999年第2期22-24,63-64,共5页Medical Journal of Chinese People’s Health

基　　金：河南省科委科技攻关资助

摘　　要：目的为探讨ＣｙｃｌｉｎＤ１、ＣＤＫ４基因及蛋白在食管癌发生中的意义及其相互间的作用。方法采用ＬＳＡＢ免疫组化染色法，对４３例食管癌手术标本的ＣｙｃｌｉｎＤ１、ＣＤＫ４蛋白进行标记。结果在鳞癌组织中，两种抗体的阳性物质存在于细胞核中和／或胞浆中，在正常鳞状上皮主要存在于核中，且从正常鳞状上皮→癌旁不典型增生→鳞癌组织，ＣｙｃｌｉｎＤ１、ＣＤＫ４的阳性率逐渐上升，统计学处理：正常上皮与癌组织之间的阳性率有差异显著性（ＰＣｙｃｌｉｎＤ１＜０．０５，ＰＣＤＫ４＜０．０５）；但未检出ＣｙｃｌｉｎＤ１、ＣＤＫ４与癌分化的关系。另外，在正常鳞状上皮中，ＣｙｃｌｉｎＤ１、ＣＤＫ４则多集中在基底层的增殖带。结论ＣｙｃｌｉｎＤ１、ＣＤＫ４蛋白表达的变化是食管癌发生中的早期事件，并且在正常细胞周期中起正调控作用。Abstract In order to study the role of CyclinD 1、CDK 4 gene and protein in the carcinogesis of esophageal Cancer,as well as the interation between them.CyclinD 1、CDK 4 of the specimens from 43 cases of esophageal squamous cell carcinomas were labelled with immunohisto chemical LSAB method.The results are as fouows:The positive substance of CyclinD 1、CDK 4 were all seen in nucleus and cytoplasm in cancer tissue,they were mainly seen in nucleus in nomal epithelium;With the change from the nomal epithelium to displasis to squamous cancer tissue,the number of CyclinD + 1、CDK + 4 increased gradually.There were significant positive rate CyclinD 1、CDK 4 between the cancer tissue and the nomal epithelium (P CyclinD 1 <0 05,P CDK 4 <0 05).But there were not significant positive rate difference of CyclinD 1、CDK 4 among each grade.We also found higer positive expression rates of CyclinD 1 and CDK 4 in the spinous cells of the nomal epithelia.The Study domonstrated that the alteration of CyclinD 1、CDK 4 protein is an early event in the Carcinogenes of esophaged cancer,In addition,CyclinD 1、CDK 4 gene provide a positive control regulation for nomal cell cycle.

关 键 词：食管癌 CyclinD₁、CDK₄蛋白 免疫机制 

分 类 号：R735.102[医药卫生—肿瘤]