逆转录PCR及原位杂交检测人IgA肾病肾脏中TGF-β mRNA  

TGF β mRNA IN HUMAN IGA NEPHROPATHY EXAMINED WITH RT PCR AND IN SITU HYBRIDIZATION

作　　者：王子慧[1] 于鼎[1] 王瑞绵[1] 

机构地区：[1]香港中文大学铁道部第四勘查设计院

出　　处：《中国组织化学与细胞化学杂志》1998年第4期43-47,共5页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：为研究转化生长因子－β（ＴＧＦ－β）在ＩｇＡ肾病病理机制中的作用，本文用逆转录ＰＣＲ（ＲＴ－ＰＣＲ）法及原位杂交法检测ＩｇＡ肾病患者肾活检组织中的ＴＧＦ－βｍＲＮＡ。结果证实：在人正常肾和ＩｇＡ肾病肾组织中均可检测到ＴＧＦ－βｍＲＮＡ的ＲＴ－ＰＣＲ扩增产物；ＴＧＦ－βｍＲＮＡ原位杂交的阳性信号主要分布于肾小管和集合管上皮细胞内，肾小球内的阳性信号较弱，且强度与系膜的增生程度有关。In order to study the effects of transforming growth factor β(TGF β) on the pathogenesis of IgA nephropathy the TGF β mRNA in kidney tissues from biopsy of IgA nephropathy was examined with the methods of reverse transcription (RT) PCR and in situ hybridization. The rersults confirmed that: 1) the RT PCR product of TGF β mRNA was found in the kidney tissues of normal and IgA nephropathy. 2)The positive signal of TGF β mRNA revealed by in situ hybridization mainly distributed in epithelial cells of renal tubule and collecting tubule. The positive signal in glomerulus was weaker than that in tubules, and the intensity of signal was related to the level of proliferation of mesangium.

关 键 词：TGF-Β 逆转录PCR 原位杂交 IGA肾病 人 

分 类 号：R361[医药卫生—病理学]