Regulation of noncoding region for expression of Sendai virus hemagglutinin-neuraminidase (HN) gene  

作　　者：胡建红 齐义鹏 

出　　处：《Science China(Life Sciences)》1999年第4期362-369,共8页中国科学（生命科学英文版）

摘　　要：Hemagglutinin-neuraminidase (HN) protein was expressed in COS-7 cells, indicating that the expression of HN protein driven by SRα promoter is higher than that driven by chicken β-actin promoter. Moreover, with 5' noncod-ing region (NCR) of HN gene, the expression was enhanced. Northern blotting demonstrated that this phenomenon was caused by the difference of HN mRNA transcription. To know the regulatory function of 5' NCR, HN gene 5' NCR was replaced by 5' NCR of keratin gene or cytochrome P-450 gene and the 3' NCR was deleted by site-directed mutagenesis. By using CAT gene as a reporter, SI nuclease assay was done to quantitate the HN mRNA transcript in the COS-7 cells co-transfected with the reporter and mutated plasmids, indicating that 5' NCR is non-specific to the enhancement of HN protein expression, and the 3' NCR also has a special regulatory function.Hemagglutinin-neuraminidase (HN) protein was expressed in COS-7 cells, indicating that the expression of HN protein driven by SRα promoter is higher than that driven by chicken β-actin promoter. Moreover, with 5’ noncoding region (NCR) of HN gene, the expression was enhanced. Northern blotting demonstrated that this phenomenon was caused by the difference of HN mRNA transcription. To know the regulatory function of 5’ NCR, HN gene 5’ NCR was replaced by 5’ NCR of keratin gene or cytochrome P-450 gene and the 3’ NCR was deleted by site-directed mutagenesis. By using CAT gene as a reporter, S1 nuclease assay was done to quantitate the HN mRNA transcript in the COS-7 cells co-transfected with the reporter and mutated plasmids, indicating that 5’ NCR is non-specific to the enhancement of HN protein expression, and the 3’ NCR also has a special regulatory function.

关 键 词：Sendai VIRUS HEMAGGLUTININ-NEURAMINIDASE REGULATION for expression. 

分 类 号：R346[医药卫生—基础医学]