rhIL-3增强急性髓性白血病细胞对柔红霉素敏感性的实验研究  

Experimental Study on Sensitivity of Acute Myeloblast Leukemia Cells to Daunomycin Enhanced by Recombinant Human Interleukin-3

作　　者：王景昌 王庆余[1,2] 

机构地区：[1]解放军第10医院 [2]第三军医大学新桥医院

出　　处：《西北国防医学杂志》1998年第3期5-8,共4页Medical Journal of National Defending Forces in Northwest China

摘　　要：目的：观察重组人白细胞介素３（ｒｈＩＬ３）刺激后ＡＭＬ细胞对柔红霉素（ＤＮＲ）敏感性的变化。方法：利用流式细胞仪（ＦＣＭ）、细胞形态学、联苯胺染色，观察ｒｈＩＬ３对ＡＭＬ细胞增殖与分化的影响；用荧光分光光度法测定ＡＭＬ细胞内ＤＮＲ浓度；用半固体培养法观察ＣＦＵＡＭＬ产率。结果：①ｒｈＩＬ３对体外培养的ＡＭＬ细胞具有促增殖作用，能增强ＡＭＬ细胞对ＤＮＲ的敏感性；②ｒｈＩＬ３或ｒｈＩＬ３联合ＤＮＲ对ＡＭＬ细胞无明显诱导分化作用；③ｒｈＩＬ３不影响ＡＭＬ细胞对ＤＮＲ的摄取与排泄平衡。结论：本实验为ｒｈＩＬ３与ＤＮＲ联合应用。Purpose: To evaluate the effect and the mechanism of recombinant human interleukin-3(rhIL-3) on the sensitivity of acute myeloblast leukemia(AML) cells to daunomycin(DNR). Methods: The effects of rhIL-3 of the proliferation and differentiation on AML cells were observed through the flow cytometry(FCM), cyto-morphology classification and benziaine dyeing. The intracellular concentration of DNR in AML cells was measured through fluorospectrophotometry. The CFUAML cell existing rate was countered through semisolid cultivation. Results: rhIL-3 can induce AML cells into cytoproliferation cycle and enhance its sensitivity to DNR, but it had no obvious effect on inducing differentiation of AML cells, rhIL-3 didn't break the balance between AML cells absorbing and excreting DNR. Conclusion: The combined using of rhIL-3 and DNR established an experimental base for increasing the clinical therapeutical effectiveness to AML treating.

关 键 词：白细胞介素-3 急性髓性白血病 柔红霉素 

分 类 号：R733.71[医药卫生—肿瘤]