食管癌端粒酶活性的检测  

THE TEL OMERASE ACTIVITY IN HUMAN ESOPHAGEAL CARCINOMA

作　　者：张登学[1] 张翠萍[1] 顾华丽[1] 张琪[1] 丁荣生[1] 刘海灵[2] 

机构地区：[1]青岛医学院附属医院急症科,青岛266003 [2]中国医学科学院肿瘤研究所

出　　处：《齐鲁医学杂志》1998年第4期241-242,共2页Medical Journal of Qilu

基　　金：青岛市科委资助课题! (批准号 :97-10 )

摘　　要：1目的　探讨食管癌端粒酶活性检测的临床意义。 2方法　建立并应用以 PCR技术为基础的端粒重复序列扩增 ,对 36例食管癌及其癌旁组织进行检测。 3结果　 36例食管癌组织中 2 9例端粒酶活性呈阳性 ,阳性检出率为 80 .5 0 % ;癌旁组织中 2例呈阳性 ,阳性检出率为 6 .0 0 % ,两者相比差异具有极显著性 (χ2 =41.30 ,P<0 .0 0 1)。36例食管癌均为鳞状细胞癌 ,其中 17例伴随淋巴结转移的标本中端粒酶活性均呈阳性 ,而在 19例未伴随淋巴结转移的病人中仅有 12例检测出端粒酶的活性 ,其活性检出率为 6 3.15 % ,两者相比差异具有显著意义 (P=0 .0 0 6 )。 4结论　端粒酶活性检测可作为食管癌诊断的指标之一 。Objective To evaluate the role of telomerase activity in human esophageal carcinoma. Methods By using PCR-based telomeric repeat amplification protocol assay,telomerase activity was examined in 36 specimens of esophageal carcinoma (EC) and their adjacent tissues obtained from patients who underwent surgical resections. Results It was found that telomerase activity was positive in 2 9of the36 esophageal carcinoma specimen(80 .5 0 % ) and in2 of the36 adjacent tissue specimen (6 .0 0 % ) (χ2 =41.2 0 ,P<0 . 0 0 1) ;the positive rates of telomerase activity in 17EC with metastasis in lymph nodes and in 19EC without metstasis in lym ph nodes were 10 0 % and 6 3. 15 % respectively(P=0 .0 6 ) . Conclusion It suggested that activity of telomerase might be a diagnostic marker of EC,and played an important role in prognosis of EC.

关 键 词：食管肿瘤 端粒酶 聚合酶链反应 

分 类 号：R735.1[医药卫生—肿瘤]