多药耐药基因表达与急性髓细胞性白血病化疗效果的关系  

CL INICAL REL EVANCE OF MDR-1/P170 EXPRESSION IN ACUTE MYEL OBL ASTIC LEUKEMIA

作　　者：曹庆学[1] A.Schulz P.Engling C.Scheid V.Diehl PD.Wickramanayake 

机构地区：[1]青岛医学院附属医院血液科,青岛266003 [2]UniversityHospitalCologne,Germany

出　　处：《齐鲁医学杂志》1998年第4期252-254,共3页Medical Journal of Qilu

摘　　要：1目的　探讨白血病细胞多药耐药 (MDR)基因表达与化疗效果的关系。 2方法　应用 MRK- 16和C1 2 9单克隆抗体对 2 0例急性髓细胞性白血病 (AML)进行了流式细胞术、细胞免疫化学、多聚酶链反应检查 ,并行细胞遗传学分析。 3结果　 18例初、复诊病人均有 MDR表达 ,但 MDR表达与完全缓解 (CR)率无线性关系 ,化疗未完全缓解者 CD+ 3 4细胞增多。 4结论　 MDR基因表达难估 AML 预后 ,CD+ 3 4细胞可作为 AMLObjective To study the relationship between the expression of multidrug resistance gene in leukemic cells and the result of chm otherapy. Methods Expression of P170 encoded by the MDR-1gene were investigated by FACS(MRK- 16 ) ,RT-PCR ,imm unocytochemistry(C1 2 9) and cytogenetics respectvely in 2 0 patients with acute myeloblastic leukemia. Results 18out of2 0 clinical spcimens expressed MDR-1gene products.There was no correlation between MDR-1gene expression and CR.However,we found a highier level of CD+ 3 4positivity in those who failed in achievement of CR after chemotherapy. Conclusion The results implied that CD+ 3 4,not MDR gene expression was associated with a decreased CR rate.

关 键 词：白血病 髓母细胞性 药物耐受性 基因表达调节 多聚酶链反应 流式细胞术 

分 类 号：R733.71[医药卫生—肿瘤]