角质形成细胞生长因子(KGF)在喉粘膜良性、癌前及恶性病变中的mRNA水平分析  

ANALYSIS OF KERATINOCYTE GROWTH FACTOR (KGF) mRNA LEVEL IN BENIGN, PREMALIGNANT AND MALIGNANT LARYNGEAL EPITHELIA

作　　者：邓志宏[1] 黄维国[1] 金岩 TipoeGL WhiteFH 

机构地区：[1]第四军医大学西京医院耳鼻喉科,西安710032 [2]口腔医学院病理科,西安710032 [3]香港大学医学院解剖学系

出　　处：《中国组织化学与细胞化学杂志》1998年第2期222-225,250,共5页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：利用原位杂交的方法检测KGFmRNA在正常喉粘膜上皮（N）、慢性非特异性炎症（IF）、不典型增生（DYS）及鳞癌（SCC）中的转录水平，探讨KGF在喉粘膜良性及恶性病变中的分布和可能的作用。结果表明，KGFmRNA不仅在间质中的成纤维细胞中表达，少量的炎细胞及血管内皮细胞中亦表达，而且从N、IF、DYS到SCC、KGFmRNA转录水平逐渐增强；上皮细胞及肿瘤性上皮细胞不表达KGFmRNA，KGFmRNA在分化差的SCC周围间质中表达较分化好的SCC周围间质增多。结论：KGF在上皮与间充质细胞的交互作用中发挥着重要的作用，对维持喉粘膜正常结构、代谢及喉癌的发生发展具有重要意义。The localization and transcriptional level of keratinocyte growth factor (KGF ) mRNA in normal (N), inflammatory (IF), dysplasia (DYS) and squamous cellcarcinoma (SCC ) were observed by in situ hybridization in order to determine the distribution and effects of KGF in normal and benign conditions and the carcinogenesis of laryngeal mucosa. The results showed that KGF mRNA was expressed by fibroblasts, endothelial cells and inflammatory cells, but not by any of the epithelial cells. There was an increasing tendency of mRNA transcriptional lever from N, IF, DYS to SCC. Furthermore, the expression level was higher in poorly differentiated SCC than in well differentiated SCC. It is concluded that KGF plays an important role in the interaction between epithelial cells and mesenchymal cells. KGF might be one of the critical factors for maintaining the structure and metabolism of llormal mucosaand the growth of laryngeal carcinoma.

关 键 词：KGF 喉粘膜 癌 原位杂交 

分 类 号：R730.2[医药卫生—肿瘤]