人肺鳞癌和腺癌组织中c-erbB-2癌基因蛋白表达与细胞增殖及预后的关系  

THE RELATIONSHIP OF THE EXPRESSION OF c-erbB-2 IN HUMAN LUNG SQUAMOUS CELL CARCINOMA AND ADENOCARCINOMA WITH CELL PROLIFERATION AND PROGNOSIS

作　　者：陈寿松[1] 赫丽[1] 许冬生[1] 蒋红卫[1] 肖同浩[1] 

机构地区：[1]广州军区武汉总医院,武汉430070

出　　处：《中国组织化学与细胞化学杂志》1998年第2期226-230,共5页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：用S－P免疫组织化学方法，用c－erbB－2多克隆抗体及PCNA单克隆抗体对50例肺鳞癌和30例腺癌组织石蜡包埋标本进行检测。结果显示：c－erbB－2染色总阳性率为46．25％，鳞癌阳性率为40％，腺癌阳性率为56．67％，两者之间无显著性差异。伴有淋巴结转移的肺癌组织中c－erbB－2阳性表达率较高（66．67％），并且与肺癌预后有关。PCNA表达阳性级别与患者的术后生存期呈负相关（P＜0．05），与c－erbB－2表达阳性级别呈正相关（P＜0．05）。本研究提示，用免疫组织化学方法检查，c－erbB－2蛋白和PCNA共同表达可作为临床判断肺鳞癌和腺癌预后的较好指标之一。Using anti-c-erbB-2 multiclonal antibodies and PCNA monoclonal antibodies,the expression of c-erbB-2 and PCNA was investigated by streptavidin peroxidase conjugated (s-p)immunohistochemical method in paraffin-embeded specimens of 50 squamous cell carcinoma and 30 adenocarcinoma of lung. The results showed that the total positive rate was 46. 25%for c-erbB-2, of which the c-erbB-2 positive rate of squamous cell carcinoma was 40% and adenocarcinoma was 56. 67%. There was no significant correlation between squamous cell carcinoma and adenocarcinoma. The c-erbB-2 positive rate was 66. 67% in patients with lymph node metastasis, which was related to the prognosis of the tumor. The positive grading of PCNA was negatively correlated with the survival time after operation (P＜0. 05 ).and it was positively correlated with the positive grade of c-erbB-2 (P<0. 05). The results suggest that using immunohistochemical method, the expression of both c-erbB-2 and PCNA was one of useful markers to judge the prognosis of squamous cell carcinoma and adenocarcinoma of lung.

关 键 词：肺癌 C-ERBB-2 PCNA 免疫组织化学 

分 类 号：R734[医药卫生—肿瘤]