寒冷对大鼠下丘脑核团癌基因c-fos及垂体ACTH细胞表达的变化  

EFFECT OF COLD STIMULATION ON THE EXPRESSION OF PROTO ONCOGEN C FOS OF HYPOTHALAMUS AND ACTH CELLS OF ANTERIOR PITUITARY IN THE RAT

作　　者：袁德霞[1] 董兰凤[1] 苏丽英[1] 康文华[1] 赵春芳[1] 

机构地区：[1]河北医科大学组织胚胎学教研室

出　　处：《中国组织化学与细胞化学杂志》1998年第1期24-27,共4页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：本实验用寒冷刺激大鼠，观察垂体ＡＣＴＨ细胞及下丘脑核团ｃ－ｆｏｓ癌基因表达蛋白出现的变化。发现寒冷能促使垂体前叶ＡＣＴＨ细胞数目增多，体积胀大，且具有粗大突起伸到扩张的血窦旁。在视交叉腹外侧的视上核（ＳＯ）神经元及视交叉上核（Ｓｃｈ）周围部神经元皆出现ｃ－ｆｏｓ强阳性反应。第三脑室侧壁室管膜上皮及少数室周核（ｐｅ）神经元为ｃ－ｆｏｓ阳性。视前内侧区（ＭＰＡ）可见分散的阳性神经元。位于室旁核内的外侧，出现ｃ－ｆｏｓ反应较浅的小神经元。本文最后探讨有关ＡＣＴＨ释放的调节。The changes of expression of proto ancogen c fos in the hypothalamus and ACTH cells in the pituitary by stimulation of cold using immunohistochemstry were observated. The expression of c fos protein in neurons of supraoptic nucleus (SO) and suprachiasmatic nucleus(SCH) appeared strong immunoreactiv. However, The parvocellular part of paraventricular hypothalamic nucleus(PVN) showed weak positive reaction. But the periventricular nucleus (PE), medial preoptic area(MPA) and tanycytes were mediate immunoreactive. The cytoplasma of ACTH cell presented brown black colour and the nucleus of these cells were coloureless. The number and volume of ACTH cells were more increased than control group in anterior pituitary and these cells with longer processes tended to be enlarged sinusoid in expermental group.

关 键 词：原癌基因C-FOS ACTH细胞 视上核 免疫组织化学 寒冷刺激 大鼠 

分 类 号：R392.11[医药卫生—免疫学]