β-半乳糖苷酶基因在正常人及白血病人骨髓细胞的表达  

THE EXPRESSION OF LACZ GENE IN BONE MARROW CELLS OF NORMAL PERSONS AND LEUKENMIA PATIENTS

作　　者：陆华中[1] 邹萍[1] 李崇渔[1] 

机构地区：[1]同济医科大学血液病学研究所

出　　处：《中国组织化学与细胞化学杂志》1998年第1期43-46,共4页Chinese Journal of Histochemistry and Cytochemistry

基　　金：卫生部科学研究基金

摘　　要：为探索组织化学方法检测标志基因在正常骨髓细胞及白血病细胞的表达，本研究利用脂质体介导将ＮｅｏＲ基因和ＬａｃＺ基因共转移正常人及白血病人骨髓细胞。然后，采用Ｘ－ｇａｌ染色的方法，我们观察了ＬａｃＺ基因在转导细胞的表达。结果显示：ＬａｃＺ基因在正常人及白血病人骨髓细胞表达的阳性率分别为１３．３３％±２．６８％和１５．３９±３．６９％。经Ｇ４１８筛选后，转导阳性细胞率达到４６．０６％±３．４７％和４８．２２％±４．４７％。提示：经脂质体介导ＬａｃＺ基因在正常骨髓细胞及白血病细胞可获得高效的转移率。同时表明，利用组织化学方法可有效地检测标志基因的表达。In trying to detect the expression of marker gene in bone marrow cells and leukemic cells by using histochemic staining, we have transduced NeoR gene and LacZ gene co transfer into bone marrow cells of normal persons and leukemia patients mediated by liposome. Then by means of X gal staining, we have observed the expression of LacZ gene in transduced cells. The results show that the transduced positive rate of lacZ gene in the bone marrow cells of normal persons and leukemia patients is 13 33%±2 68% and 15 39%±3 69% respectively. By the selection of G418, the rate of transduced positive cells reaches to 46 06%±3 47% and 48 22%±4 47%. It indicates that the high efficiency of LacZ gene transfer in normal bone marrow cells and leukemic cells mediated by liposome can be obtained. Meanwhile, the expression of marker gene in these cells can be effectively detected by histochemical staining.

关 键 词：基因转移 基因标志 骨髓细胞 白血病 Β-D-半乳糖苷酶 

分 类 号：R733.7[医药卫生—肿瘤]