老年性学习记忆减退大鼠基底前脑NOS神经元的形态变化  

THE MORPHOLOGICAL CHANGES OF NOS NEURONS IN THE BASAL FOREBRAIN OF AGED LEARNING AND MEMORY DEFICIT RAT

作　　者：王怀星[1] 姚志彬[1] 顾耀铭[1] 陈以慈[1] 

机构地区：[1]中山医科大学解剖学教研室脑研究室

出　　处：《中山大学学报（医学科学版）》1997年第S1期6-9,13+1,共6页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家教委博士点基金

摘　　要：用Ｍｏｒｉｓ水迷宫行为检测方法，以青年组平均逃避潜伏期加２倍标准差为下限，加１倍标准差为上限将老年大鼠分为学习记忆减退组和学习记忆正常组。取受试大鼠的前脑冰冻切片，行尼克酰胺腺嘌呤二核苷酸磷酸（ｎｉｃｏｔｉｎａｍｉｄｅａｄｅｎｉｎｅｄｉｎｕｃｌｅｏｔｉｄｅｐｈｏｓｐｈａｔｅ，ＮＡＤＰＨ）组化染色。结果显示：３７．５％的老年鼠为学习记忆减退鼠。与青年鼠相比，老年学习记忆减退鼠基底脑一氧化氮合酶（ｎｉｔｒｉｃｏｘｉｄｅｓｙｎｔｈａｓｅ，ＮＯＳ）神经元胞体萎缩，着色变淡，突起减少。而老年学习记忆正常鼠基底脑内ＮＯＳ神经元形态正常。ＩＢＡＳ图象分析表明老年学习记忆减退鼠基底前脑各核团ＮＯＳ神经元数、细胞面积和灰度值较青年鼠明显下降（Ｐ＜０．０１）。而老年学习记忆正常鼠上述指标无明显改变。受试大鼠逃避潜伏期与基底前脑ＮＯＳ神经元数量呈负相关。上述结果提示，老年大鼠学习记忆能力减退存在着明显的个体差异。老年学习记忆减退大鼠基底前脑ＮＯＳ神经元发生严重的退变，这可能是老年性学习记忆减退的神经机制之一。Forty aged(26 month old) rats were divided into learning and memory deficits group and normal group according to the mean escape latency of young(3 month old) rats in Morris water maze task.Forebrain freezing microtome tissue sections were stained by NADPH histochemical technique.The result showed that 37.5% of old rats were learning and memory deficit rats,and theirs NOS neurons in basal forebrainshrunked,the dendrites decreased,compared with the youngs.The old learning and memory normal rats showed no obviously changes.IBAS image system was used for quantity analysis.The cell number,the cell area and the gray level of NOS neurons in the basal forebrain of the aged deficits obviously decreased compared with the youngs( P <0.01).The escape latencies of the tested ratrs correlated inversely with the NOS neuron numbers in the basal forebrain.The results indicated that the changes of NOS neurons in basal forebrain is one of the nerve mechanism of aged learning and memory deficits.

关 键 词：前脑/细胞学 学习障碍/病理学 记忆障碍/病理学 神经元/细胞学 大鼠 衰老 

分 类 号：R749.1[医药卫生—神经病学与精神病学]