铝对大白鼠海马所致损害的实验研究  

EXPERIMENTAL STUDY OF INJURY EFFECT OF ALUMINUM ON THE HIPPOCAMPAL OF RAT

作　　者：熊希凯[1] 董大翠[1] 张艳[1] 王家兵 陈艳贤[1] 朱清华[1] 章锡平[1] 梁杏兰[1] 

机构地区：[1]同济医科大学解剖学教研室,营养卫生学教研室

出　　处：《中国组织化学与细胞化学杂志》1997年第4期7-9,11,共4页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金

摘　　要：为探讨铝对海马的损害，用３０只健康大白鼠，体重２００～３００克，分为Ａ组（正常对照）１０只，Ｂ组（低铝组饲料中加５００ｍｇ／ｋｇＡｌＣｌ３）１０只，Ｃ组（高铝组饲料中加２５００ｍｇ／ｋｇ／ＡｌＣｌ３）１０只。分别饲养１２个月后，分组断头取脑海马，按组织化学及酶组织化学常规做“铝定性”反应，琥珀酸脱氢酶（ＳＤＨ）、乳酸脱氢酶（ＬＤＨ）、硫胺素焦磷酸酶（ＴＰＰａｓｅ）、镁激活三磷酸腺苷酶（Ｍｇ２＋－ＡＴＰａｓｅ）、胆碱酯酶（ＣｈＥ）测定。按电镜常规做超微结构的观察。结果提示：Ｃ组，海马ＳＤＨ、Ｍｇ２＋－ＡＴＰａｓｅ、ＣｈＥ活性明显减弱（由强阳性（）下降为阳性（＋），铝反应增强（在海马的神经细胞内强阳性（）；同时海马神经元内线粒体嵴断裂、肿胀、空泡样变，神经纤维髓鞘变性（如不规则卷曲、松散）。30 Wistar rats (200～300 g) were divided into 3 groups, each group has 10 rats. Group A (10 for normal control) was given standard rat feeding, Group B (low aluminum) was fed with food material containing 500 mg/kg ALCL3, and Group C (high aluminum) fed with food material containing 2500 mg/kg ALCL3. After 12 months they were killed respectively. The changes of the enzyme histochemistry and ultrastructure in the rat hippocampal were observed. The results showed that the activities of SDH, ChE, Mg2+ATPase in hippocampal cells were decreasing in growp C while the aluminum reaction was increasing in the cytoplasm of hippocampal eclls. The ultrastructural changes indicated that there was brisement of the mitochordria crista, degeneration of nerve myelin sheaths. It suggests that aluminum can result in hippocampal cell damages both in function and structure in Group C.

关 键 词：铝 酶组织化学 超微结构 大鼠 

分 类 号：R363[医药卫生—病理学]