肝移植对肝硬化大鼠脾脏功能的影响  

Effects of Liver Transplantation on Spleen Function of Hepatic Cirrhosis Rats

作　　者：黄陈[1] 高涌[1] 谈燚[1] 刘金新[1] 李宗狂[1] 刘会春[1] 崔培元[1] 

机构地区：[1]安徽省蚌埠医学院附属医院胆肝外科,233004

出　　处：《实用全科医学》2004年第4期285-287,共3页Applied Journal Of General Practice

基　　金：安徽省教育厅优秀青年科研项目 ( 2 0 0 2jq14 4)

摘　　要：目的　探讨肝硬化大鼠肝移植前后脾脏功能变化。方法　制备四氯化碳中毒性肝硬化大鼠模型 ,采用“二袖套法”进行肝移植。观察肝移植前后大鼠外周血细胞数的变化。采用反相高效液相色谱法检测肝移植前后大鼠血清中促吞噬肽 (Tuftsin)含量的变化。结果　肝硬化大鼠外周血细胞数显著降低 ,血清Tuftsin含量明显减少。肝硬化大鼠肝移植后 ,随着时间延长 ,大鼠外周血细胞数明显回升 ;血清Tuftsin含量也开始增加 ,并随时间推移呈逐渐上升趋势。结论　肝移植可使因肝功能严重损害而导致的脾脏功能障碍得到改善。Objective To investigate the changes of spleen function before and after liver transplantation in hepatic cirrhosis rats. Methods Hepatic cirrhosis model rats were established by injecting carbon tetrachloride subcutaneously. Liver transplantation model was established with two-cuff technique. The blood cell number in peripheral blood was observed before and after liver transplantation in hepatic cirrhosis rats. The serum tuftsin level was detected by reverse-phase high performance liquid chromatography (RP-HPLC) before and after liver transplantation. Results Before liver transplantation, the cell number of peripheral blood and the level of serum tuftsin decreased significantly. After liver transplantation, the cell number of peripheral blood increased significantly. Meanwhile, the serum tuftsin level was increased with time prolongation. Conclusions Liver transplantation can improve the splenic dysfunction caused by hepatic dysfunction.

关 键 词：肝移植 肝硬化 大鼠 脾脏功能 肝功能 

分 类 号：R575.2[医药卫生—消化系统] R-332[医药卫生—内科学]