MM3工程菌的大规模发酵培养工艺研究  

Fermentation studies of recombinant Escherichia coli MM3 in large scale

作　　者：王叙甫[1] 方宏清[1] 陈明[1] 王焕金 谢妍 阎明凡[1] 张群伟[1] 冯尔玲[1] 于公义[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100071

出　　处：《生物技术通讯》1997年第1期17-19,共3页Letters in Biotechnology

摘　　要：首先在50L发酵罐上研究了MM3工程菌的发酵培养工艺。确定了接种量、搅拌转速、pH和补料速率等参数,活菌数可达每毫升211亿。以溶氧为放大准则可成功地将该工艺在200L国产发酵罐上再现。说明该工艺具有可放大性,可在全国各药厂推广应用。Fermentation studies of recombinant Escherichia coli MM3 as a live vaccine were first performed in a 50L fermentor. After determining inoculum volume, agitation speed, pH and feed speed, the colony-forming unit in ferment broth was reached at 211 ×108/ml. This culture process could be successfully scaled up in a 200L fermentor based on oxygen supply.

关 键 词：疫苗 大肠杆菌 发酵 大规模 

分 类 号：Q939.9[生物学—微生物学]