Effects of Cholate on the Stability of Bacteriorhodopsin in Alkaline Solution  

作　　者：王居硕 许强 赵南明 

出　　处：《Tsinghua Science and Technology》1997年第1期74-77,共4页清华大学学报（自然科学版（英文版）

摘　　要：The effects of cholate on the structure of bacteriorhodopsin (bR) in the purple membrane and the effects of alkali on native and cholate treated bR were studied using absorption spectra, fluorescence spectra and CD spectra. Treatment mass fraction of 10% cholate at room temperature for 48 h had no obvious effect on the structure of bR, yet changed the composition of the purple membrane, making cholate treated bR more stable in alkaline solution than native bR.The effects of cholate on the structure of bacteriorhodopsin (bR) in the purple membrane and the effects of alkali on native and cholate treated bR were studied using absorption spectra, fluorescence spectra and CD spectra. Treatment mass fraction of 10% cholate at room temperature for 48 h had no obvious effect on the structure of bR, yet changed the composition of the purple membrane, making cholate treated bR more stable in alkaline solution than native bR.

关 键 词：bacteriorhodopsin (bR) purple membrane CHOLATE absorption spectra fluorescence spectra CD spectra 

分 类 号：Q734[生物学—分子生物学]