外源性野生型p53基因在K562－n细胞表达的意义  

EXPRESSION OF EXOGENEOUS WILD-TYPE p53 GENE IN K562-n CELLS

作　　者：许小平[1] 丁训杰[1] 阎影[1] 史剑慧 程文英 冯凯 张安胜 

机构地区：[1]上海医科大学附属华山医院血液研究室,中国人民解放军总医院,上海医科大学放射医学研究所

出　　处：《中国癌症杂志》1996年第2期96-99,共4页China Oncology

摘　　要：目的研究外源性野生型ｐ５３基因在红白血病细胞表达的意义。方法采用重组逆转录病毒载体介导的方法将人野生型ｐ５３基因ｃＤＮＡ转移入Ｋ５６２－ｎ细胞，用形态学、流式细胞仪和联苯胺染色试验检测转基因后的Ｋ５６２－ｎ细胞。结果野生型ｐ５３基因可以诱导Ｋ５６２－ｎ细胞编程性细胞死亡和分化的形态特征，且伴有细胞周期Ｇ１期的生长阻滞。结论野生型ｐ５３基因能抑制人红白血病细胞生长的某些机制。PURPOSE In order to study the significance of expression of exogeneous wild-type p53 gene erythroleukemia cells.METHODS Wild-type p53 gene cDNA was transferred by recombinant retroviral vector into K562-n cells and transfer gene K562-n cells were examined by morphology, flow cytometry and benzidine staining methods.RESULTS It was found that exogeneous wild-type p53 gene could induce K562-n cells to die. The dead cells presented morphological features of apoptosis. Flow cytometric analysis showed p53 gene induced K562-n cell death and was accompanied by a growth arrest in the G1 phase of the cell cycle. In addition,benzidine staining indicated that wild-type p53 gene could induce some K562-n cells to undergo differentiation.CONCLUSIONS Wild-type p53 gene suppressed the growth of human erythroleukemia cells by inducing apoptosis and differentiation.

关 键 词：基因.p53 基因转移 逆转录病毒 白血病 

分 类 号：R394.2[医药卫生—医学遗传学]