SPC－A－1／DDP耐药细胞株的建立及其生物学特性的初步观察  

ESTABLISHMENT OF SPC-A-1/DDP CELL LINE OF DDP RESISTANCE AND PRELIMINARY OBSERVATION ON ITS BIOLOGICAL CHARACTERISTICS

作　　者：陆婉琴[1] 

机构地区：[1]上海医科大学附属肿瘤医院

出　　处：《中国癌症杂志》1996年第3期162-165,共4页China Oncology

摘　　要：目的建立耐药细胞株，观察其生物学特性。方法采用体外诱变和药压的方法，建立耐顺铂（ＤＤＰ）的人肺腺癌细胞株ＳＰＣ－Ａ－１的耐药株（ＳＰＣ－Ａ－１／ＤＤＰ）。结果ＳＰＣ－Ａ－１／ＤＤＦ耐药细胞株能耐受的ＤＤＰ浓度为耐ＤＤＰ细胞株的１．５～１５倍，且细胞增殖和传代及耐药性均甚稳定。其倍增时间为３４．３５±０．３５３６ｈ，细胞被抑制５０％时的药物浓度（ＩＣ５０）为４５μｇ／ｍｌ，耐药指数为５．６２５，集落形成率为５３．９７％±２．４９３２％。结论ＳＰＣ－Ａ－１／ＤＤＰ耐药株可以作为研究抗癌药物耐药性机制的实验模型。To establish drug resistant cell line and observe its biological characteristics.METHODS Using mutagen MNNG and cultured in high concentration of DDP, a human lung adenocarcinoma cell line SPC-A-1/DDP resistant to DDP was established.RESULTS The concentration of DDP that SPC-A-1/DDP drug resistant cell line could resist was 1. 5to 15 times that of the DDP resistant cell line. Its cell proliferation and generative as well drug resistantproperties were very stable. The doubling times of SPC-A-1/DDP and SPC-A-1 were 34. 35+0. 3536 h and33. 1 +0. 2828 h respectively. When 50% of cells were inhibited, the drug concentration(IC_(50)) was 45 μg/ml, and the drug resistance index(RI)was 5. 625, the colony formation rate was 53. 97% +2. 4932%.CONCLUSIONS SPC-A-1/DDP cdll line might be used as an experimental model for the study of the mechanism of anticancer drug resistance.

关 键 词：肺肿瘤 腺癌 细胞株 耐药性 诱变剂 顺铂 

分 类 号：R73-3[医药卫生—肿瘤]