肺癌细胞AgNOR计数与DNA含量测定之间相关性研究  

A STUDY OF RELATIONSHIP BETWEEN NUCLEOLAR ORGANIZED REGIONS AND DNA CONTENT IN LUNG CANCER

作　　者：倪洪珍[1] 曹世龙[1] 施达仁[1] 潘文生[1] 黄抗美[1] 李焕蓉 李栋良[1] 沈瑾[1] 周决[1] 蒋娉[1] 张凤坤[1] 

机构地区：[1]上海医科大学附属肿瘤医院,上海医科大学附属中山医院

出　　处：《中国癌症杂志》1996年第4期251-253,共3页China Oncology

摘　　要：目的研究肺癌细胞ＤＮＡ倍体类型和核仁组织区嗜银蛋白（ＡｇＮＯＲ）及两者之间的相互关系。方法对３６例肺癌石蜡标本进行细胞ＡｇＮＯＲ定量计数和ＤＮＡ含量的流式细胞术（ＦＣＭ）检测。检测结果进行ｔ检验和Ｘ２检验。结果二倍体肺癌细胞ＡｇＮＯＲ计数（１．６５±０．８７）显著低于异倍体肺癌细胞者（３．０９±１．０３）细胞周期中Ｓ期细胞＜２０％的肺癌细胞ＡｇＮＯＲ计数（１．３８±１．０２）显著低于Ｓ期细胞≥２０％者（２．７７±１．１４）。在二倍体肿瘤中，分化Ⅱ级和Ⅲ级细胞的ＡｇＮＯＲ计数显著高于分化Ⅰ级者，但在Ⅱ、Ⅲ级之间ＡｇＮＯＲ计数则无显著差异；分化Ⅲ级肿瘤的异倍体检出率显著高于分化Ⅰ、Ⅱ级肿瘤，但在Ⅰ、Ⅱ级之间异倍体检出率则无显著差异。结论细胞ＤＮＡ含量和ＡｇＮＯＲ计数与肺癌细胞的增殖状态、恶性程度及预后有密切相关性；在病理分级中以上两种检测具有互补性。The aim of this project is to investigate the relationships between DNA content and AgNOR in lung cancer.METHODS 36 cases of paraffin-embedded lung cancer specimens were analysed by FCM for DNA contents and AgNOR count. X2 was used for statistical study.RESULTS The AgNOR counts were 3.09±1.03 in lung cancer with aneuploid DNA contents which were significantly higher than the contents obtained from lung cancer (1.65±0.87) with diploid DNA content (P<0.05). On the other hand, the cells in S phase less than 20% showed AgNOR counts lower than the amount with S phase greater than 20%. In diploid tumors, cells with gradeⅡand Ⅲ differentiation had higher AgNOR counts than gradeⅠcells, while there was no significant difference in AgNOR counts between cells with gradeⅡand Ⅲ differentiation. CONCLUSIONS There is a marked correlation between cellular DNA content and AgNOR count to proliferation status, degree of malignancy and prognosis; the two methods of examination are reciprocally helpful and might be a useful indicator for prediction in lung cancer.

关 键 词：肺肿瘤 核仁组织区 脱氧核糖核酸 二倍性 异倍性 流式细胞计量术 

分 类 号：R734.[医药卫生—肿瘤]