人肝细胞癌中N－ras、c－myc癌基因的表达──原位分子杂交和免疫组织化学研究  

EXPRESSIONS OF N-ras c-myc ONCOGENE IN HUMAN HEPATOCELLULAR CARCINOMA-AN IN SITU HYBRIDIZATION AND IMMUNOHISTOCHEMICAL STUDY

作　　者：苏勤[1] 张劲风[1] 刘彦仿[1] 

机构地区：[1]第四军医大学唐都医院病理科,第四军医大学唐都病理学教研室,大连军医学校病理学教研室

出　　处：《中国组织化学与细胞化学杂志》1995年第2期129-133,231,共6页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金;军队"八五"医疗卫生科研(青年)基金

摘　　要：为了明确Ｎ－ｒａｓ、ｃ－ｍｙｃ癌基因在人肝细胞癌（ＨＣＣ）中的表达及其形态学分布，我们对３８例石蜡包埋的ＨＣＣ（其中３３例带有癌周肝组织）标本进行了原位核酸杂交和免疫组化研究。结果表明，ＨＣＣ及癌周肝组织中Ｎ－ｒａｓ表达阳性率分别为４２％和３９％；ｃ－ｍｙｃｍＲＮＡ阳性表达率分别为４７％和３６％；ｃ－ｍｙｃ蛋白阳性率分别为５３％和３９％。ＨＣＣ与癌周肝之间Ｎ－ｒａｓ、ｃ－ｍｙｃｍＲＮＡ及ｃ－ｍｙｃ蛋白表达水平无显著差别；ｃ－ｍｙｃｍＲＮＡ及蛋白阳性强度也无显著差别，其形态学分布基本一致，主要集中于癌细胞和癌周肝中的部分高度增生结节；Ｎ－ｒａｓ表达增强主要见于癌细胞、癌周肝中的高度增生结节、小细胞性不典型增生及少部分肝小多角细胞。它们与ＨＢｘＡｇ表达之间无明确的对应关系。上述结果显示，ＨＣＣ中Ｎ－ｒａｓ、ｃ－ｍｙｃ表达都显著增强，它们可能是人ＨＣＣ发生中较晚期的事件，而Ｎ－ｒａｓ表达增强早于ｃ－ｍｙｃ癌基因活化。An in situ hibridization and immunohistochemical study was made on the expressions ofN-ras,c-myc oncogene as well as their histologic distribution in 38 cases of human hepatocellular carcinoma(HCC)tissue in which Thirty-three cases were with pericancerous livertissues. esults shosed N-ras mRNA hibridization signals were found in 42% of HCCs and39% of the pericancerous liver tissues and C-myc mRNA positive signals was foind in 47%ofHCCs and 36% of the pericancerous tissues C-myc protein immunoreactive products were observed in 53% of HCCs and 39% of the pericancerous tissues. No significant difference wasfound between the expression levels of N-ras mRNA,c-myc mRNA and C-myc protein inHCCs and in the pericancerous liver tissues. Moreover,good correlation was found betweentheexpressions of C-myc mRNA and protein and their histologic distribution both in HCCsand in the pericancerous liver tissues. C-myc mRNA and protein was localized mainly in thecancer cells and hyperplastic nodules in the pericancerous liver tissues. Hyperexpression ofN-ras mRNA, c-myc mrNA was observed maninly in the cancer cells and the hyperlpasticnodules, small-cell dyplasia as well as a small number of small polygonal liver cells in thepericancerous tissues. No definite correlation was found between the expressions of N-ras orc-myc mRNA and HBxAg.These results demonstrated that expressions of N-ras and c-mycincreased significantly in HCC compared with normal liver.It is suggested that N-ras and c-myc hyperexpression may he rather late events, compared with IGF Ⅱ gene reactivation, inthe course human hepatocarcion-genesis, The hyperexpression of N-ras may be earlier thanthat of c-myc.

关 键 词：肝细胞瘤 人 基因 肿瘤 核酸杂交 免疫组织化学 

分 类 号：R735.7[医药卫生—肿瘤]