中国树鼩下丘脑视上核和室旁核内加压素能和催产素能神经元的形态计量学分析  

MORPHOMETRIC ANALYSIS OF VASOPRESSINERGIC AND OXYTOCINERGIC NEURONS IN THE SUPRAOPTIC AND PARAVENTRICULAR NUCLEI OF HYPOTHALAMUS IN TUPAIA BELANGERI CHINENSISL

作　　者：刘能保[1] 张敏海[1] 刘少纯[1] 戴晓莉[1] 周顺长[1] 

机构地区：[1]武汉同济医科大学组织胚胎学教研室,德国萨尔州大学解剖学研究所,同济医科大学动物实验中心VP能神经元,OT能神经元,视上核,室旁核,形态计量学分析,中国树鼩

出　　处：《中国组织化学与细胞化学杂志》1995年第1期64-70,共7页Chinese Journal of Histochemistry and Cytochemistry

基　　金：德国大众汽车公司基金;同济医科大学科研处资助

摘　　要：采用免疫组织化学ＰＡＰ／ＤＡＮ－镍加强技术和形态计量法研究了中国树鼩下丘脑加压素（ＶＰ）能和催产素（ＯＴ）能视上核主部（ＳＯＮｐ）、视上核交叉后部（ＳＯＮｒ）和室旁核（ＰＶＮ）的平均矢轴长度，ＳＯＮｐ，ＳＯＮｒ和ＰＶＮ内ＶＰ能和ＯＴ能神经元胞体截面积的平均值；ＳＯＮｐ，ＳＯＮｒ和ＰＶＮ的ＶＰ能和ＯＴ能神经元总数；各校团的各个系列节段内ＶＰ能和ＯＴ能神经元的比较计数。发现，ＳＯＮｐ前１／３部分ＯＴ能神经元明显多于ＶＰ能的，而后２／３部分则相反；ＳＯＮｒ前２／３部分ＶＰ能神经元明显多于ＯＴ能的，而后１／３部分两者差别不明显；ＰＶＮ前２／３部分ＯＴ能神经元明显多于ＶＰ能的，而后１／３部分则相反。本文就中国树下丘脑ＳＯＮ和ＰＶＮ内ＶＰ能和ＯＴ能神经元的形态计量学资料与大鼠的进行了比较。A morphometric analysis of vasopressin(VP)-ergic and oxytocin(OT)-ergic neurons in the supraoptic(SON) and paraventricular nuclei(PVN) of the hypothalamus in Tupaia Belangeri Chinensis was conducted by using the immunohistochemical nickel-reinforced PAP/DAB technique and the morphometry.It comprised the mean rostrocaudal extents of VP-and OT-ergic principal part of the SON(SONp) retrochiasmatic SON(SONr) and PVN; the average area of section of the perikarya of the VP-and OT-ergic neurons in the SONp,the SONr and the PVN, the total number of the VP-and OT-ergic neurons in the SONp, the SONr and the PVN,and the comparative count of the VP-and OT-ergic neurons in each serial segment of each nerve nucleus.It was found that the OT-ergic neurons in the rostral 1/3;of the SONp were considerably more numerous than VP-ergic ones,while the picture of the posterior 2/3 of the SONp was contrary to that of the rostral 1/3.the VP-ergic neurons in the rostral 2/3 of the SONr were remarkably more numerous than the OT-ergic neurons,however,the difference between the VP-and OT-ergic neurons in number was unremarkable in the posterior 1/3 of the SONr;the OT-ergic neurons in the rostral 2/3 of the PVN were obviously more numerous than the VP-ergic ones while the picture of the posterior 1/3 of the PVN was opposite to that of the rostral 2/3.In this paper the comparison of the morphometric data of the VP-and OT-ergic neurons in the SON and PVN-of the hypothalamus in Tupaia Belangeri Chinensis to those in rats was also discussed.

关 键 词：VP能神经元 OT能神经元 视上核 室旁核 形态计量学分析 中国树鼩 

分 类 号：Q57[生物学—生物化学]