环磷酰胺(CTX)所致的骨髓损伤与恢复中血清粒、巨噬细胞集落刺激活性(CSA)的变化  

THE CHANGE OF SERUM COLONY-STIMULATING ACTIVITY LEVEL DURING CYCLOPHOSPHAMIDE INDUCED MYELO-DEPRESSION AND ITS RECOVERY IN MICE

作　　者：李惠平[1] 汪有蕃[1] 贾廷珍[1] 徐勤 孙武 

机构地区：[1]北京医科大学第三附属医院职业医学研究中心放射医学科,北京100083

出　　处：《中国实验血液学杂志》1994年第1期73-77,共5页Journal of Experimental Hematology

摘　　要：本实验报道了注射CTX的小鼠,骨髓粒系造血抑制及其恢复过程中,血清CSA水平的动态变化。三组实验小鼠的CTX剂量分别为150mg/kg、250mg/kg、350mg/kg 1次腹腔注射,于注射后第1、3、5、9、13、17、24天常规方法观察外周血白细胞,骨髓有核细胞(BMC)计数,同时用微量半固体琼脂培养方法观察粒、巨噬祖细胞(CFU-GM),收集血清作粒、巨噬细胞集落刺激活性测定。结果表明,给CTX后,血清CSA分别于第3～5天开始升高,第9天达峰值,三个组分别为正常对照组的186％、226％、208％(p<0.001),持续9～10天,而在同一时期所观察的骨髓造血的各项指标已恢复到正常的80～90％,表明化疗药物引起的骨髓造血损伤恢复中,血清CSA起了重要的调节作用。实验结果证明,监测血清CSA水平对判断粒系造血的损伤程度和恢复有一定价值。取血清进行测定在临床上具有可行性,它将有助于阐明各种治疗措施的临床效果与CSA水平的关系,对探讨化疗药物与外源性GM-CSF联合应用方案可提供实验依据。因而值得进一步深入研究。For the purpose of using a simple and convenient way such as the serum CSA assay to assess the CFU-GM function and its recovery ability during hematopoietic suppression we determined the serum CSA level of mice intoxicated with CTX. Mice were divided into four groups: (1) normal control, (2) three CTX groups (150mg/kg, 250mg/kg and 350mg/kg, respectively). On days 1, 3, 5, 9, 13, 17, 24 after CTX intoxication blood samples were taken from 5 mice of each group at random for determination of WBC, bone marrow cellularity and CFU-GM as well as CSA level. The results demonstrated that the serum CSA in three CTX groups began to increase on 3rd or 5th day after intoxication and reached their own highest values on day 9(186%, 226%, 208% of normal control respectively P< 0.001), all of which remained above the value of normal control for 9-10 days while all the indices of granulepiesis were well recovered (80-90% of normal control). The results indicated that CSA may play an important role in the recovery of hematopoietic depression caused by CTX intoxication and the CSA assay may be a valuable and practicable way in the assessment of granulopoietic function and its recovery ability. In addition, the results may be useful for the clinical study of the integration of cytotoxic drugs and growth factors.

关 键 词：环磷酸胺 骨髓造血抑制 集落刺激活性 

分 类 号：R363[医药卫生—病理学]