贵州苗族新生儿HbF中G_γ/A_γ值测定与A_γT基因频率分析  

Ratioes of Gy and Ay Chains,and the Analysis of AyT Gene Frequency in Miao Nationality Newborns in Guizhou Province

作　　者：易志勤[1] 南国华[2] 陈松森[3] 梁植权[3] 姚源黎 杨文鸿 

机构地区：[1]赣南医学院生化学教研室 [2]江西医学院生化学教研室 [3]中国医学科学院 [4]贵州凯里妇幼保健院

出　　处：《赣南医学院学报》1992年第1期1-4,共4页JOURNAL OF GANNAN MEDICAL UNIVERSITY

摘　　要：用含TritonX-100的酸性尿素聚丙烯酰胺凝胶电泳(PAGE)法测定了贵州省苗族108例新生儿脐带血胎儿血红蛋白(HbF)中Gγ/Aγ值,其中男性新生儿64例,女性新生儿44例。显示107例(占99.07％)新生儿的Gγ值在55％～78％之间,高Gγ值者1例(占0.925％)。均值与标准差为70.42±3.45。用Large-pore Vydac C_4柱反相HPLC法对108例样本进行了AγT基因频率(fAγT)分析,fAγT为0.0648。The Gr to Ar ratioes of fetal hemoglobin in 108 cord blood samples of Miao nationality newborns in Guizhou province was determined by the polyacrylamide gel electrophoresis.The result showed that a case has high Gr value (0.925%) and 107 babies have the normal Gr values of 55 to 78%(70.42± 3.45).The ArT Gene Frequcy (fArT) analysis in 108 samples was done by high pressure liquid chromatography using a large pore Vydac C4 column,the fArT was 0.0648.

关 键 词：血红蛋白F Gγ/Aγ值 AγT基因频率 聚丙烯酰胺凝胶电泳 高效液相层析 苗族 

分 类 号：R[医药卫生]