大鼠组织中超氧阴离子自由基的提取和化学发光测定  

The Extraction and Chemiluminescence Determination of Superoxide Free Redical in Tissues of Experimental Animals

作　　者：周宜开[1] 郝巧玲[1] 袁津玮[1] 万有[2] 任恕[1] 

机构地区：[1]同济医科大学环境医学研究所 [2]同济医科大学基础医学部

出　　处：《华中科技大学学报（医学版）》1992年第S1期1-3,共3页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：在通氮条件下,以四丁基氯化铵(TBAC)为稳定剂,用去水除氧的二甲基亚砜(DMSO),从大鼠新鲜组织中提取超氧阴离子自由基(O_2^-),并运用化学发光法进行检测.提取最佳时间为3min。发光体系pH为8。测定结果表明。大鼠不同组织中(O_2^-)含量(cpm/g·ml)顺序为肺>肾>肝>脑。Using tetrabutyl ammonium chloride (TBAC) and under continuous nidro-gen.superoxide free radicals (O2-) were extracted from the fresh tissues of Wistar rats with oxygen-and water free dimethyl sulphoxide (DMSO).The contents of (O2-) in the extracts were determined by chemiluminescence analysis. The time of extraction is 3 min and the optimum pH in the luminescent reaction system is 8. The results show that the distribution sequence of the contents of (O2-) in different tissues of rats (cpm/g · ml) is lung>kidney> liver>brain.

关 键 词：活性氧 超氧阴离子自由基 化学发光分析 

分 类 号：R[医药卫生]