新疆不同来源细粒棘球蚴原头节DNA杂交分析  

An Analysis of DNA Hybridization of Protoscolices of Echinococcus granulosus from Different Sources in Xinjiang

作　　者：李新兰[1] 张文林[1] 瞿群[1] 焦伟[1] 柴君杰[1] 

机构地区：[1]新疆地方病防治研究所

出　　处：《疾病预防控制通报》1992年第S1期58-61,共4页Bulletin of Disease Control & Prevention(China)

基　　金：中华人民共和国卫生部科研基金

摘　　要：用曼氏血吸虫核糖体 RNA 基因的 BamHI 酶解片段之一 pSM889做探针,对新疆不同来源的细粒棘球绦虫原头节 DNA 进行限制性酶切片段长度多态性的分析。结果显示新疆的南疆(和田)和北疆(阿勒泰)地区羊源细粒棘球绦虫样本之间以及羊源、牛源和人源样本之间的区别不明显,骆驼源细粒棘球绦虫样本与羊源、牛源及人源样本之间存在着明显的区别,骆驼源细粒棘球绦虫 DNA 经内切酶 EcoRI、BamHI、Hind Ⅲ和 PistⅠ消化后分别与探针 pSM889杂交产生的图型均不同于其他样本。这一差异的生物学和流行病学意义有待进一步的研究。此结果对新疆细粒棘球绦虫虫株问题的研究将提供有力的依据。An analysis of DNA restriction fragment length polymorphisms of rRNA genes was made on protoscolices of E.garnulosus of different origin in Xinjiang,using pSM889,one of the BamHI restriction fragments of rRNA genes of Schistosoma mansoni, s probe. Ex tion of the probed EcoRI,BamHI,Hind Ⅲ and Pist Ⅰ restriction fragments revealed no clear distinction in hybridization banding patterns between E.granulosus isolates of sheep origin from South Xinjiang(Hotan)and from North Xinjiang(Altay)and among isolates of sheep, cattle and human origin but clear distinction between E.granulosus isolates of camel origin and of sheep,cattle and human origin.The probed hybridization banding patterns produced by DNAs of camel E.granulosus isolates after digestion with restriction endonucleases EcoRI, BamHI,Hind Ⅲ and Pist Ⅰdiffered all from those of other isolates.The biological and epidemiolgical significance of this difference remains to be studied further.The findings will provide strong evidence for study on E.graulosus strains in Xinjiang.

关 键 词：细粒棘球绦虫 DNA 杂交 DNA 探针 pSM889 虫株 新疆 

分 类 号：R[医药卫生]